Gemini sigma 300 vp

Manufactured by Zeiss

Sourced in Germany

The Gemini Sigma 300 VP is a high-performance scanning electron microscope (SEM) developed by Zeiss. It is designed for materials science and industrial applications, providing users with advanced imaging and analytical capabilities. The Gemini Sigma 300 VP utilizes a field emission electron gun and a variable pressure mode, allowing it to examine a wide range of sample types, including non-conductive and outgassing specimens, without the need for extensive sample preparation.

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Lab products found in correlation

Em ace600, by Leica (1 mentions) Jsm 7500f, by JEOL (1 mentions)

Close spelling variants of this product

Sigma 300 (401 citations) Gemini 300 (212 citations) Sigma VP (144 citations) Sigma 300 VP (47 citations) Gemini Sigma 300 (9 citations) Gemini Sigma 300 VP SEM (2 citations)

4 protocols using gemini sigma 300 vp

Scaffold Structure and hMSC Morphology Evaluation

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Scaffold structure and hMSC morphology were evaluated for each culture protocol after 30 h post differentiation by scanning electron microscopy (SEM) (Thermo Fischer Scientific, FEI Apero VS, Darmstadt, Germany). The samples were fixed in 4% formaldehyde, dehydrated in a serial dilution of ethanol, dried in tert-butanol, and immediately freeze-dried. Prior to imaging, all samples were sputter-coated (Leica, EM ACE600) with a 2 nm film of platinum to ensure conductivity of the sample’s surface. Images were taken at an accelerating voltage equal to 1.5–3 kV and a magnification of ×200 and ×5000. Cellular details were artificially colored on magnified images using Photoshop^® CS6 (Adobe, v13.0.1) for visualization purposes.
In addition, a dispersive energy X-ray (EDS) detector (Carl Zeiss, Gemini Sigma 300 VP, Oberkochen, Germany) operating at 10 KeV was used to determine the surface atomic composition of the decellularized bone scaffolds. Three random areas of interest were evaluated for each sample.

Pereira A.R., Lipphaus A., Ergin M., Salehi S., Gehweiler D., Rudert M., Hansmann J, & Herrmann M. (2021). Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions. Materials, 14(16), 4431.

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Surface Characterization of Zirconia Ceramics

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The surface nanostructure of the blank-machined zirconia, self-glazed zirconia, and coated zirconia surfaces was characterized by using a scanning electron microscope (SEM, Gemini Sigma 300/VP, Zeiss, Oberkochen, Germany). The samples were washed with distilled water and acetone in an ultrasonic bath and dried at room temperature. The SEM observation was carried out on the surfaces. Accelerating voltages of 1 and 2 kV were applied in order to reduce the charging up of the samples.

Wu J., Yu P., Lv H., Yang S, & Wu Z. (2021). Nanostructured Zirconia Surfaces Regulate Human Gingival Fibroblasts Behavior Through Differential Modulation of Macrophage Polarization. Frontiers in Bioengineering and Biotechnology, 8, 611684.

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Geochemical Analysis of V-Bearing Deposits

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Nineteen
samples for this research were collected from the V-bearing
deposits at the Bagong section, Sansui, Guizhou Province. The sample
used for elemental analysis was ground to 200 mesh powder, then fully
mixed with Li₂B₄O₇-LiBO₂ flux, and melted at 1000 °C. After the molten material was
cooled, dilute HNO₃ and dilute HCI were added for dissolution,
and then analysis by X-ray fluorescence spectroscopy (XRF) was performed.
The type of instrument used for analysis is PANanalytical PW2424.
The content of some elements was also analyzed by inductively coupled
plasma mass spectrometry (ICP-MS). The analysis was conducted at ALS
Chemex (Guangzhou) Co. Ltd.
The data of TOC are calculated on
loss on ignition (LOI). The conversion
relationship between LOI and TOC is:
We used 2.125 as the conversion
factor between LOI and TOC in this
study; this conversion factor is based on the study of Qiu et al.⁵⁵Samples with high V content (samples shyc-2,
shyc-6, and shyc-7)
were picked out for scanning electron microscopy (SEM) and energy
dispersive spectroscopy (EDS) analyses. These samples were treated
by desiccation and gold spraying, SEM was used for the analysis of
morphology, and EDS was used for determination of chemical elements
in different minerals. The model of SEM-EDS was Zeiss Gemini Sigma
300 VP, and all samples were scanned in Guizhou University.

Wu T., Yang R., Gao L., Li J, & Gao J. (2021). Origin and Enrichment of Vanadium in the Lower Cambrian Black Shales, South China. ACS Omega, 6(41), 26870-26879.

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Characterizing MOF Film Sorption Properties

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Surface morphology and surface coverage were investigated by a field emission scanning electron microscopy (FE-SEM, ZEISS Gemini Sigma 300 VP). Cross-sectional SEM images were measured by a JEOL JSM-7500F operated in Gentle Beam mode without sputtering in order to determine the film thickness. Sorption properties of the MOF films were carried out on an environmental-controlled QCM (BEL-QCM-4 instrument, MicrotracBEL Corp.) at controlled temperature of 25 °C using methanol as probe molecules. Prior to the sorption measurements, the films were activated in-situ within the BEL-QCM instrument by purging with a dry He gas with a flow rate of 100 sccm for 2 h until the change of QCM frequency was stable within the range of ±5 Hz in 20 min. Then the mass of the MOF film was recorded by a conversion of the difference between the QCM frequency at the final activation and the fundamental frequency of the SAM-functionalised QCM substrates according to Sauerbrey’s equation. After that, methanol sorption isotherms at 25 °C were collected by varying P/P₀ of saturated methanol vapour in He gas flow from 0.0 to 95.0%.

Wannapaiboon S., Schneemann A., Hante I., Tu M., Epp K., Semrau A.L., Sternemann C., Paulus M., Baxter S.J., Kieslich G, & Fischer R.A. (2019). Control of structural flexibility of layered-pillared metal-organic frameworks anchored at surfaces. Nature Communications, 10, 346.

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