Briefly, the tibias and femurs of the mice were isolated, the muscle removed and the ends of the bones were cut off. The bone marrow was collected by rinsing the shaft with 2-3 ml PBS per bone using a 25 g needle. The bone marrow suspension was aliquoted and shock-frozen in liquid nitrogen. Samples were thawed, sonicated 3-times for 10 seconds each using a tip sonicator (Ultrasonic GE50) to achieve complete lysis and homogenization of the samples. The aliquots were analyzed using ELISA for cytokines (CCL5, SDF-1, IL-6, Osteopontin, IL-10, TNF-α, IL-17, IL-23), arginine, PGE2 and arginase-1 concentrations. ELISA assays were performed exactly following the instructions of the vendors. The mouse CCL-5 ELISA kit was from Abcam (# ab100739), mouse CCR5 ELISA from Aviva Systems Biology (# OKEH 03441), mouse Arginase-1 ELISA from Abcam (# ab269541), mouse SDF-1 ELISA from Abcam (# ab100741), mouse Osteopontin ELISA from Abcam (# ab100734), mouse IL-6 ELISA kit from R&D (# M6000B), mouse IL-1 ELISA from R&D (# MLB 00C), mouse IL-10 ELISA from R&D (# M1000B), mouse IL-17 ELISA from R&D (# M1700), mouse IL-23 ELISA from R&D (# M2300), mouse TNFα ELISA from R&D (# MTA 00B) and the PGE2 ELISA from R&D (# KGE004B). Arginine was quantified using a commercial assay from abcam (# ab241028). All values were normalized to protein in the samples.
Mouse il 17 elisa
Manufactured by R&D Systems
The Mouse IL-17 ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse interleukin-17 levels in cell culture supernates, serum, and plasma.
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Lab products found in correlation
2 protocols using mouse il 17 elisa
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Bone Marrow Cytokine and Metabolite Analysis
2
Bone Marrow Cytokine and Metabolite Analysis
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Briefly, the tibias and femurs of the mice were isolated, the muscle removed and the ends of the bones were cut off. The bone marrow was collected by rinsing the shaft with 2-3 ml PBS per bone using a 25 g needle. The bone marrow suspension was aliquoted and shock-frozen in liquid nitrogen. Samples were thawed, sonicated 3-times for 10 seconds each using a tip sonicator (Ultrasonic GE50) to achieve complete lysis and homogenization of the samples. The aliquots were analyzed using ELISA for cytokines (CCL5, SDF-1, IL-6, Osteopontin, IL-10, TNF-α, IL-17, IL-23), arginine, PGE2 and arginase-1 concentrations. ELISA assays were performed exactly following the instructions of the vendors. The mouse CCL-5 ELISA kit was from Abcam (# ab100739), mouse CCR5 ELISA from Aviva Systems Biology (# OKEH 03441), mouse Arginase-1 ELISA from Abcam (# ab269541), mouse SDF-1 ELISA from Abcam (# ab100741), mouse Osteopontin ELISA from Abcam (# ab100734), mouse IL-6 ELISA kit from R&D (# M6000B), mouse IL-1 ELISA from R&D (# MLB 00C), mouse IL-10 ELISA from R&D (# M1000B), mouse IL-17 ELISA from R&D (# M1700), mouse IL-23 ELISA from R&D (# M2300), mouse TNFα ELISA from R&D (# MTA 00B) and the PGE2 ELISA from R&D (# KGE004B). Arginine was quantified using a commercial assay from abcam (# ab241028). All values were normalized to protein in the samples.
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