The results of ciprofloxacin, clindamycin, trimethoprim‐sulfamethoxazole (TMP‐SMX), quinupristin‐dalfopristin (Q‐D), erythromycin, fusidic acid, gentamicin, mupirocin, nitrofurantoin, penicillin, G‐D, rifampicin, tetracycline, tigecycline, and linezolid were obtained with the automated system Vitek2 (bioMérieux). The minimal inhibitory concentration (MIC) of vancomycin was determined using the vancomycin E test (AB Biodisk) on Mueller‐Hilton agar. All the results of antimicrobial susceptibility were interpreted according to CLSI guideline.18
Vancomycin e test
Manufactured by bioMérieux
Sourced in United States
The Vancomycin E test is a diagnostic tool used in clinical microbiology laboratories. It is designed to determine the minimum inhibitory concentration (MIC) of vancomycin, an antibiotic commonly used to treat infections caused by Gram-positive bacteria. The test provides quantitative information about the susceptibility of bacterial isolates to vancomycin, which is essential for guiding appropriate antimicrobial therapy.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using vancomycin e test
1
Antimicrobial Susceptibility Testing Methods
2
Molecular Characterization of MRSA Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All isolates were confirmed as MRSA by polymerase chain reaction (PCR) for the mecA gene, and tested for antimicrobial susceptibility by standard techniques according to Clinical and Laboratory Standards Institute guidelines [23 ]. Staphylococcal cassette chromosome (SCC) mec type MRSA was identified [24 (link)]. The presence of bacterial virulence factors, including adhesins and toxins, was examined by multiplex PCR [25 (link),26 (link)], and multi-locus sequence typing was also performed [27 (link)]. To examine agr dysfunction, the extent of δ-hemolysin production was measured by streaking each MRSA isolate next to a β-hemolysin disk (Remel, Lenexa, KS, USA) [28 (link)]. The vancomycin minimum inhibitory concentrations (MICs) were determined using the vancomycin E-test (AB Biodisk, Piscataway, NJ, USA) on Mueller-Hinton agar. Heteroresistant vancomycin-intermediate S. aureus was identified by a modified population analysis profile-area under curve ratio method [29 (link)].
3
Molecular Profiling of MRSA Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MRSA isolates were collected from the microbiology laboratory of Kaohsiung Medical University Hospital at the time of blood culture identification and stored at -80°C. All S. aureus isolates were identified and tested for susceptibility to antimicrobial agents using the Vitek 2 system (bioMeriéux, Marcyl’Etoile, France). The interpretation of susceptibility followed Clinical and Laboratory Standards Institute (CLSI) guidelines [14 ]. Vancomycin MICs were determined by the Vancomycin E-test (AB Biodisk, Solna, Sweden) using a 0.5 McFarland inoculum streaked evenly with a swab onto Mueller-Hinton agar plates [18 (link)].
A multiplex polymerase chain reaction (PCR) method was used for SCCmec typing, and multilocus sequence typing (MLST) was performed as described in previous studies [19 (link), 20 (link)]. MLST and SCCmec types were further inferred for all strains. The PCR method described by Gilot P. et al. was used to determine accessory gene regulator (agr) typing [21 (link)].
A multiplex polymerase chain reaction (PCR) method was used for SCCmec typing, and multilocus sequence typing (MLST) was performed as described in previous studies [19 (link), 20 (link)]. MLST and SCCmec types were further inferred for all strains. The PCR method described by Gilot P. et al. was used to determine accessory gene regulator (agr) typing [21 (link)].
4
Vancomycin Susceptibility of MRSA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All S. aureus isolates were identified using standard methods. Methicillin resistance was confirmed by polymerase chain reaction (PCR) detection of the mecA gene. Vancomycin MICs of methicillin-resistant S. aureus (MRSA) isolates were determined by the Vancomycin Etest (AB Biodisk, Piscataway, NJ, USA) on Mueller-Hinton agar according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!