Antichicken dylight 488

Manufactured by Abcam

Antichicken DyLight 488 is a fluorescently labeled secondary antibody targeting chicken primary antibodies. It is conjugated to the DyLight 488 fluorescent dye, which has an excitation maximum of 493 nm and an emission maximum of 518 nm.

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Lab products found in correlation

Vectashield mounting medium, by Vector Laboratories (1 mentions) Fluoview fv10i confocal microscope, by Olympus (1 mentions) Fv10 asw software, by Olympus (1 mentions) Alexa fluor 568 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Flag m2 monoclonal antibody, by Merck Group (1 mentions) Antimouse dylight 488, by Vector Laboratories (1 mentions) Anti rabbit dylight 488, by Vector Laboratories (1 mentions)

3 protocols using antichicken dylight 488

Immunofluorescence Analysis of Viral Proteins

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DAOY and A549 cells were seeded in chamber slides (0.3 cm²/well; 1 × 10⁴ cells/well) and at the indicated time intervals p.i. processed as previously described (59 (link)). As the primary antibodies, guinea pig anti-TBEVC (1:1500 dilution), mouse anti-FLAG tag (1:200 dilution), rabbit anti-NPM1 (1:200 dilution), mouse anti-flavivirus E (1:200 dilution), chicken anti-NS3 (1:5000 dilution), mouse anti-dsRNA J2 (1:250 dilution), rabbit anticalnexin (1:200 dilution), and rabbit anti-COX4 (1:200 dilution) were used. As the secondary antibodies, anti-rabbit DyLight 488 (1:500 diluiton), antichicken DyLight 488 (Abcam; 1:500 dilution), antiguinea pig Alexa Fluor 594 (1:500 dilution), antimouse DyLight 594 (1:500 dilution), and antimouse DyLight 488 (1:500 dilution) were used. Slides were eventually mounted in Vectashield mounting medium (Vector Laboratories). The Olympus Fluoview FV10i confocal microscope was used for imaging, and images were exported in FV10-ASW software (Olympus Life Science; version 1.7).

Selinger M., Novotný R., Sýs J., Roby J.A., Tykalová H., Ranjani G.S., Vancová M., Jaklová K., Kaufman F., Bloom M.E., Zdráhal Z., Grubhoffer L., Forwood J.K., Hrabal R., Rumlová M, & Štěrba J. (2022). Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural–biological analysis. The Journal of Biological Chemistry, 298(11), 102585.

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Multicolor Flow Cytometry Panel

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Anti-CD11c (HL3), anti-CD19 (1D3), anti-B220 (RA3-6B2), anti-CD11b (M1/70) mAbs were obtained from BD Pharmingen, San Diego CA. Anti-PDCA1 (120G8) was a gift of Georgio Trinchieri (formerly at Schering-Plough). Chicken anti-Sendai, anti-chicken DyLight 488 or 594, sheep anti LC3 were purchased from AbCam, Cambridge MA. Anti-Chicken HRP was purchased from Thermo Scientific, Waltham, MA. Donkey anti-Rabbit alexa-fluor 568, donkey anti sheep alexa- fluor 647 were purchased from Life Technologies, Eugene, OR. Anti-MOMA1 and anti-MARCO were purchased from AbD Serotech, Kidlington, UK.

Flores M., Chew C., Tyan K., Huang W.Q., Salem A, & Clynes R. (2015). FcγRIIB prevents Inflammatory Type I Interferon Production from pDCs during a Viral Memory Response. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4240-4250.

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Comprehensive Antibody Panel for Viral Protein Detection

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The following primary antibodies were used: anti-TBEVC polyclonal antibody (23 ), monoclonal FLAG M2 antibody (Sigma–Aldrich; catalog no.: F1804-200UG), anti-NPM1 polyclonal antibody (Life Technologies; catalog no.: PA5-12446), anti-COX4 monoclonal antibody (Life Technologies; catalog no.: MA5-15078), anti-calnexin monoclonal antibody (Abcam; catalog no.: ab22595), Anti-E Flavivirus Group Antigen Antibody (Merck Millipore; catalog no.: MAB10216), anti-dsRNA mAb SCICONS J2 (Biocompare; catalog no.: 10010200), anti-TBEV NS3 polyclonal antibody (Dr M. Bloom, NIAID; (23 )), anti-RPL9 antibody (EP13752) (Abcam; catalog no.: ab182556), anti-KAP1/TRIM28 antibody (Abcam; catalog no.: ab190178), anti-OASL Polyclonal Antibody (Life Technologies; catalog no.: PA5-31317), anti-CSDE1/NRU antibody (EPR17413) (Abcam; catalog no.: ab200663), and anti-MYBBP1A antibody (Abcam; catalog no.: ab99361).
The following secondary antibodies were used: goat anti–guinea pig horseradish peroxidase (HRP) (Life Technologies; catalog no.: A18769), anti-rabbit DyLight 488 (Vector Laboratories; catalog no.: DI-1488), antichicken DyLight 488 (Abcam; catalog no.: ab96947), anti–guinea pig Alexa Fluor 594 (Abcam; catalog no.: ab150188), antimouse DyLight 488 (Vector Laboratories; catalog no.: DI-2488), and antimouse DyLight 594 (Abcam; catalog no.: ab96881).

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