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2 protocols using ledene

1

Terpenoid Standards Characterization Protocol

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Terpenoid standards used in the study and their purity percentages as obtained from Sigma-Aldrich (UK) were: alloaromadendrene (-) (98%), camphene (95%), camphor (R) (95%), caryophyllene (-) (trans) (98.5%), 1,8-cineole, citral (95%), p-cymene (99%), geraniol (98%), geranyl acetate (97%), ledene (+) (95%), limonene (R) (+) (97%), linalool (±) (95%), linalyl acetate (97%), 6-methyl-5-hepten-2-one (96%), myrcene (90%), α-pinene (-) (99%), β-pinene (-) (99%), sabienene hydrate (97%), α-terpinene (85%), γ-terpinene (97%), terpinolene (95%), terpinen-4-ol (95%), α-terpineol (96%), terpinyl acetate (±) (90%) and verbenol (95%).
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2

Comprehensive Analysis of Tea Tree Oil

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Tea tree oil was purchased from Essential Oil India—SAT Group, Kannauj, India. Solvents n-hexane and acetone of analytical grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Standards (α-pinene #80-56-8, sabinene #3387-41-5, camphene #79-92-5, β-pinene #127-91-3, myrcene #123-35-3, α-phellandrene #99-83-2, α-terpinene #99-86-5, p-cymene #99-87-6, limonene #5989-27-5, 1,8-cineole #470-82-6, γ-terpinene #99-85-4, terpinolene #586-62-9, (+)-terpinen-4-ol #2438-10-0, (-)-terpinen-4-ol #20126-75-5, (+)-α-terpineol #7785-53-7, (-)-α-terpineol #10482-56-1, β-caryophyllene #87-44-5, aromadendrene #489-39-4, α-humulene #6753-98-6, ledene #21747-46-6, globulol #489-41-8, and viridiflorol 552-02-3) were from Sigma-Aldrich (St. Louis, MO, USA) and α-copaene #3856-25-5 was purchased from Fluka Chemical Co., Buchs, SG, Switzerland.
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