Ascend 600 mhz

The CO₂RR performances of the various samples were tested by using a three-electrode flow cell system in a CO₂-saturated 0.5 M KHCO₃ aqueous solution. All electrochemical measurements were conducted on a CHI 760E clectrochemical workstation (CH instrument, Shanghai, China). And, all the CO₂RR performances were measured after the stable LSV scanning. If not specified, all CO₂ reduction performance was collected without iR compensation in this work. Approximately 2 mg of catalyst deposited on a gas diffusion layer with a 1 cm² working area was used as the working electrode (WE). The Ag/AgCl electrode and platinum plate were employed as the reference electrode (RE) and counter electrode (CE), respectively. During the electrochemical measurement, CO₂ was pumped into the cathode chamber with a constant flow rate (20 mL min⁻¹). The gas products were detected by using an in situ connected gas chromatograph instrument (PANNA, A91lus). The liquid products were analyzed by nuclear magnetic resonance (NMR) spectrometer (Bruker Ascend TM 600 MHZ). The Faradaic efficiency (FE) during the CO₂RR is calculated by the equation, FE = Q_i/Q_t = (N_i × n × F)/Q_t, where Q_i is the charge amount for product reduction, Q_t is the total charge consumed, N_i is the product molar amount, n is the electrons transfer number (which is 2 for formate, H₂ and CO), and F is the Faradaic constant (96,485 C mol⁻¹).

The J–V characteristics were measured under illumination of AM 1.5 G (100 mW cm⁻²) by using a Keithley 2400 source meter. The steady‐state PL spectra and time‐resolved PL decays of the perovskite films were measured on an Edinburgh fluorescence spectrometer (FLS920) and HORIBA Scientific DeltaPro, respectively. EQE was measured on an EQE system (Enli Technology Co., Ltd.). Philips diffractometer (X'pert PRO MRD) was used to obtain the X‐ray diffraction data of perovskite films deposited on a ITO/PEDOT:PSS substrate. The morphology of perovskite films was characterized by AFM (Bruker, Dimension3100) and SEM (Carl Zeiss, GeminiSEM). The contact angles of perovskite films were measured by optical contact angle measuring and contour analysis systems (Dataphysics, OCA20). NMR was obtained by using NMR spectrometer (Bruker, Ascend TM 600 MHZ). TA spectra were performed by transient absorption spectrometer (Light Conversion, HARPIA‐TA). The thickness of the perovskite films was measured by a probe surface profiler (Bruker, DektakXT). Perovskite energy level was measured by UPS (Kratos, AXIS‐ULTRA DLD‐600W).

The ¹H and ¹³C NMR spectrum were recorded on a Bruker Ascend TM 600 MHz , and all the samples were dissolved in DMSO. The Fourier transform infrared (FT‐IR) spectra were recorded in the range 400–4000 cm⁻¹ on Bruker Alpha P spectrometer with a single reflection ATR.

Vibrio diabolicus A1SM3 was isolated from a sediment sample recovered from a Solar Saltern in Manaure, La Guajira, Colombia. This strain was deposited in the Collection of Microorganisms of Universidad de La Sabana (USAB-BIO, Chia, Colombia) registered in the RNC Colombia. The bacteria was seed in growth medium (referred as M3 medium) with the following composition: 40 g/L NaCl, 20 g/L MgSO₄ 7H₂O, 1 g/L KCl, 0.3 g/L KH₂PO₄, 0.5 g/L yeast extract, 0.5 g/L peptone (pep), 0.5 g/L casamino acids (cas), 0.5 g/L glucose (glu), 0.5 g/L starch (star), and 0.3 g/L sodium pyruvate (pyr) [41 (link)]. After 15 days of incubation at 30 °C and 150 rpm, 4L of culture broth was extracted twice with ethyl acetate (1:1) and the organic fraction was concentrated in a rotary evaporator under vacuum. The extract (110 mg) was fractionated by SiO₂ column chromatography (250 × 15 mm) using n-hexane:ethyl acetate (7:3) to ethyl acetate: methanol (9:1) gradient. A total of 80 fractions were obtained and grouped in 15 subfractions according to their TLC profiles. The grouped fractions were tested for cytotoxic activities and analyzed by HPLC-MS/MS and NMR (Bruker Ascend^TM 600 MHz, Bruker Daltonics, Billerica, MA, USA).

Vacuum-liquid chromatography (VLC) was conducted on Merck silica gel (70–230 mesh), and Medium-Pressure Liquid Chromatography (MPLC) (Biotage IsoleraTM, Uppsala, Sweden) was performed using Silica gel SNAP cartridge KP-Sil and C₁₈ SNAP cartridge KP-C18-HS (Biotage, Charlotte, NC, USA) at a flow rate of 20 mL/min. The sample separation was monitored by thin-layer chromatography (TLC). The TLC was performed on glass pre-coated silica gel 60 F254 plates (Merck, Darmstadt, Germany). Reversed-phase High-performance liquid chromatography (HPLC) was performed on a Gilson HPLC system (Gilson, Inc. Middleton, WI, USA) with a YMC C₁₈ Pro Pack 5 µm column (250 × 21.20 mm²) (YMC Co., Kyoto, Japan) at a flow rate of 6 mL/min. ¹H and ¹³C NMR, and 2D (COSY, HSQC, HMBC and NOESY) NMR spectra were recorded on a Bruker AscendTM 600 MHz (Bruker, Billerica, MA, USA). High-resolution Electrospray Ionization mass (HRESIMS) data were obtained utilizing a Synapt G2 Waters mass spectrometer (Waters, Milford, MA, USA). Optical rotations were obtained on a Jasco DIP-1000 automatic digital polarimeter (Tokyo, Japan). Circular dichroism spectrum was recorded on a Chirascan qCD (Applied Photophysics, Leatherhead, Surrey, UK).