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Nucleobases

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Nucleobases are the fundamental building blocks of nucleic acids, such as DNA and RNA. They are organic compounds consisting of a nitrogen-containing aromatic ring and a sugar molecule. The four main nucleobases are adenine, guanine, cytosine, and thymine (in DNA) or uracil (in RNA). These nucleobases play a crucial role in the storage and transmission of genetic information within living organisms.

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2 protocols using nucleobases

1

Chemical Synthesis and Characterization

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α,α’-Dibromo-ortho-xylene, α,α’-dibromo-meta-xylene, α,α’-dibromo-para-xylene, triethylamine, α-cyano-4-hydroxycinnamic acid (CCA), thymidine, nucleobases, Acyclovir, 2-phenylethanol, 1-butanol, 4-phenyl-1-butanol, deoxyribose, benzoic acid, bisphenol A, trifluoroacetic acid (TFA), and 17β-estradiol (E2) were from Sigma (St. Louis, MO). o-Xylene-d10, 99.3 atom% D, was from CDN Isotopes (Quebec, Canada). Bromine, 99.8%, was from Alfa Aesar (Ward Hill, MA). The Phenol Calibration Mix (12 components, Cat. No. 31694) was from Restek (Bellefonte, PA). Microcentrifuge tubes, pipette tips, ethyl acetate (certified ACS) and HPLC grade acetonitrile (ACN) were from Fisher Scientific (Pittsburgh, PA). All materials were used as received. CCA Matrix Solution was 5 mg/mL in 50% ACN.
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2

Synthesis of Nucleobase Nanoparticles

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Zinc acetate, cadmium acetate, sodium borohydride, mercaptosuccinic acid, nucleobases, and other used chemicals were obtained from Sigma–Aldrich (St Louis, MO, USA). High purity deionized water (Milli-Q Millipore 18.2 MΩ/cm; Millipore, Bedford, MA, USA) was used to prepare the aqueous solution of the nucleobases.
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