Anti-mouse CD34 FITC (clone RAM34), anti-mouse CD3 APC (clone145-2C11), anti-mouse CD117 (c-kit) APC (clone 2B8), and anti-mouse Ly-6 A/E (Sca1) CE/Cy7 (clone D7) were purchased from eBioscience (San Diego, CA, USA). Biotin anti-mouse CD4 (clone GK1.5), CE anti-mouse CD4 (clone GK1.5), CE anti-mouse/human CD45R/B220 (clone RA3-6B2), biotin anti-mouse/human CD45R/B220 (clone RA3-6B2), PerCP anti-mouse/human CD45R/B220 (clone RA3-6B2), PerCP anti-mouse/human CD11b (clone M1/70), FITC anti-mouse/human CD11b (clone M1/70), biotin anti-mouse/human CD11b (clone M1/70), PerCP anti-mouse Ly-6G/ Ly-6C(Gr1) (clone RB6-8C5), biotin anti-mouse Ly-6G/ Ly-6C(Gr1) (clone RB6-8C5), CE/Cy7 anti-mouse Ly-6G/Ly-6C(Gr1) (clone RB6-8C5), biotin anti-mouse Ter119 (clone TER119), FITC anti-mouse Ter119 (clone TER119), FITC anti-mouse CD8 (clone 53-6.7), biotin anti-mouse CD8 (clone 53-6.7), CE anti-mouse CD71 (clone RI7217), and PerCP streptavidin were obtained from Biolegend (San Diego, CA, USA). Anti-γH2AX rabbit monoclonal antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). FITC goat anti-rabbit IgG was obtained from ZSGB-BIO Origene (Beijing, China).
Anti mouse cd117 c kit apc clone 2b8
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-mouse CD117 (c-kit) APC (clone 2B8) is a fluorescently-labeled monoclonal antibody that specifically binds to the CD117 (c-kit) antigen expressed on mouse cells. It can be used for the identification and analysis of CD117-positive cells in flow cytometry applications.
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2 protocols using anti mouse cd117 c kit apc clone 2b8
1
Multiparameter Flow Cytometry Analysis
2
Isolation and Characterization of Bone Marrow Cells
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Total BMCs were isolated as described above. Isolated BMCs containing 2 × 106 cells were suspended in 200 μL of staining buffer (2% FBS in PBS), followed by incubation with purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™; BD Pharmingen) for 10 min at 4 °C. The following fluorescence-conjugated antibodies were then applied and incubated for 30 min at 4 °C: anti-mouse CD140a (PDGFRα APC (clone APA5; BioLegend, San Diego, CA), anti-mouse Lineage Cocktail with Isotype control APC (BD Pharmingen), anti-mouse CD117 (c-kit) APC (clone 2B8; eBioscience), and anti-mouse CD184 (CXCR4) APC (clone 2B11; eBioscience).
As matching isotype controls, fluorescence-conjugated anti-mouse IgG antibodies obtained from either BioLegend or eBioscience were used in each experiment. Stained cells were then analysed on the BD FACSCanto II system. For the sorting experiment, a BD FACSAria II was used. The sorting gates were defined based on the isotype control staining. The FACS data were analysed using FlowJo software, version 6.3.3 (Tree Star, Ashland, OR).
As matching isotype controls, fluorescence-conjugated anti-mouse IgG antibodies obtained from either BioLegend or eBioscience were used in each experiment. Stained cells were then analysed on the BD FACSCanto II system. For the sorting experiment, a BD FACSAria II was used. The sorting gates were defined based on the isotype control staining. The FACS data were analysed using FlowJo software, version 6.3.3 (Tree Star, Ashland, OR).
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