α smooth muscle actin antibody alexa fluor 488 antibody α sma

Thrombin, benzonase nuclease (DNase), and bicinchoninic acid (BCA) protein assay kit were obtained from Sigma-Aldrich. Calcein AM, Live/Dead Cell Viability Assay, glycine, bovine serum albumin (BSA), fetal bovine serum (FBS), trypsin–EDTA, Medium 200 (M200), Medium 231 (M231), Low Serum Growth Supplement kit (LSGS), Xylene Substitute Mountant, ProLong Diamond Anti-fade Mountant with DAPI, goat anti-rabbit IgG-Alexa Fluor 546 antibody, and α-smooth muscle actin antibody-Alexa Fluor 488 antibody (α-SMA) were obtained from Thermo-Fisher Scientific. Nucleic acid sequences (Table S1) were obtained from Integrated DNA Technologies (Coralville, IA). 4% paraformaldehyde solution and human Fg were obtained from Millipore (Billerica, MA). Vascular endothelial growth factor-165 (VEGF), platelet-derived growth factor-BB (PDGF-BB), VEGF enzyme-linked immunosorbent assay (ELISA) kit, and PDGF-BB ELISA kit were obtained from PeproTech (Rocky Hill, NJ). Primary rabbit anti-mouse CD31 was obtained from Cell Signaling Technology (Beverly, MA). H&E stain kit was obtained from LeicaBiosystems (Buffalo Grove, IL). Human umbilical vein endothelial cells (HUVECs) were obtained from Thermo-Fisher Scientific. Human aorta smooth muscle cells (HASMCs) were obtained from ATCC (Manassas, VA).

Calcein AM, glycine, bovine serum albumin (BSA), fetal bovine serum (FBS), medium 200 (M200), medium 106 (M106), medium 154 (M154), Low Serum Growth Supplement (LSGS), ABTS substrate, xylene substitute mountant, ProLong Diamond Antifade Mountant with DAPI, goat anti-rabbit IgG-Alexa Fluor 546 antibody, α-smooth muscle actin antibody-Alexa Fluor 488 antibody (α-SMA), and Human umbilical vein endothelial cells (HUVECs), were obtained from Thermo-Fisher Scientific. Nucleic acid sequences were obtained from Integrated DNA Technologies (Coralville, IA). Vascular endothelial growth factor-165 (VEGF) and VEGF enzyme-linked immunosorbent assay (ELISA) kit were obtained from Peprotech (Rocky Hill, NJ). 4% Paraformaldehyde was obtained from Santa Cruz (Dallas, TX). Primary rabbit anti-mouse CD31 antibody was obtained from Cell Signaling Technology (Beverly, MA). Human neonatal fibroblasts (HDFN) and mouse keratinocytes (MK) were obtained from ATCC (Manassas, VA). Hematoxylin and eosin (H&E) stain kit was obtained from Leica Biosystems (Buffalo Grove, IL). Collagenase P. was obtained from Sigma Aldrich (St. Louis, MO).