Dmem f12

Manufactured by Sartorius

Sourced in Israel, United States, China, Germany

DMEM/F12 is a cell culture medium designed for the growth and maintenance of a variety of cell types. It contains a balanced combination of nutrients, amino acids, vitamins, and other components required for cell proliferation and survival. The medium is suitable for use in a wide range of cell-based applications, including research, drug discovery, and cell-based assays.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sartorius (19 mentions) Penicillin, by Sartorius (9 mentions) Streptomycin, by Sartorius (9 mentions) L glutamine, by Sartorius (9 mentions) Penicillin streptomycin, by Sartorius (7 mentions) Dmem f12, by Merck Group (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) L glutamine solution, by Sartorius (4 mentions) Trypsin edta solution c, by Sartorius (4 mentions) Amphotericin b, by Sartorius (4 mentions) Collagenase type 2, by Worthington (4 mentions) Dulbecco modified eagle medium (dmem), by Sartorius (4 mentions) Fetal calf serum (fcs), by Sartorius (4 mentions) Dmem f12, by Thermo Fisher Scientific (4 mentions) Nutristem, by Sartorius (3 mentions) Matrigel, by BD (3 mentions) Rpmi 1640, by Sartorius (3 mentions) Trypsin, by Merck Group (3 mentions) Penicillin, by Merck Group (3 mentions) Streptomycin, by Merck Group (3 mentions)

66 protocols using dmem f12

Isolation and Culture of Liver Cells

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Livers of juvenile yellow croakers were removed and placed in sterile phosphate buffer (PBS, Biological Industries, Israel) containing penicillin and streptomycin (cat. no. P1400, Solarbio, China). After washing with Dulbecco’s modified Eagle medium/Ham’s F12 medium (1:1) (DMEM/F12, Biological Industries), the liver tissue was chopped into 1-mm³ slices and digested with 0.25% trypsin (Thermo Fisher Scientific, USA) for 10 min. After neutralization with DMEM/F12 medium containing fetal bovine serum (FBS, Biological Industries), the cell precipitate was suspended in complete medium composed of DMEM/F12 medium supplemented with 15% FBS, 100 U penicillin and 100 μg/mL streptomycin. The cell suspension was inoculated into a six-well culture plate and incubated at 28°C (Zhu et al., 2019 (link)).
HEK293T cells were maintained in DMEM with high glucose (Biological Industries) supplemented with 10% FBS and antibiotics within an atmosphere of 5% CO₂ at 37°C (Zhu et al., 2019 (link)).
AML12 cells were maintained in DMEM/F12 medium (Procell, China) containing 10% FBS, 10 μg/mL insulin, 5.5 μg/mL transferrin, 5 ng/mL selenium, 40 ng/mL dexamethasone and 1% penicillin/streptomycin at 37°C under 5% CO₂.

Chen Q., Du J., Cui K., Fang W., Zhao Z., Chen Q., Mai K, & Ai Q. (2021). Acetyl-CoA derived from hepatic mitochondrial fatty acid β-oxidation aggravates inflammation by enhancing p65 acetylation. iScience, 24(11), 103244.

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Expansion of Human iPSCs from Omental Stromal Cells

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iPSCs were generated from omental stromal cells and were a kind gift from Dr. Rivka Ofir, Ben Gurion University. The undifferentiated cells were cultivated on culture plates, pre coated with Matrigel™ (BD, Franklin Lakes, New Jersey), diluted to 250 μg/mL in DMEM/F12 (Biological Industries, Beit HaEmek, Israel). Cells were maintained in NutriStem™ (Biological Industries) medium containing 1% Penicillin/Streptomycin (Biological Industries) and cultured under a humidified atmosphere at 37°C with 5% CO₂. Medium was refreshed daily and cells were passaged weekly by treatment with 1 U/mL dispase (Stemcell Technologies, Vancouver, Canada) followed by mechanical trituration.

Gal I., Edri R., Noor N., Rotenberg M., Namestnikov M., Cabilly I., Shapira A, & Dvir T. (2020). Injectable cardiac cell microdroplets for tissue regeneration. Small (Weinheim an der Bergstrasse, Germany), 16(8), e1904806.

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Toxicological Assessment of AFB1 and ZEA

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AFB₁ and ZEA were purchased from Sigma-Aldrich (St. Louis, MO, U.S.) and dissolved in dimethyl sulfoxide (DMSO) (Shanghai Solarbio Biotechnology Co., Ltd. Shanghai, China) and 99.6% ethanol to prepare the concentrations of 1 mg/mL and 10 mg/mL as stock solutions, respectively. Work solutions were diluted with Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 at 1/1) (Hyclone, Logan, UT, U.S.) without serum and antibiotics. Phosphate-buffered saline (PBS) and thiazolyl blue tetrazolium bromide (MTT) were purchased from Solarbio (Shanghai Solarbio Biotechnology Co., Ltd. Shanghai, China). The final concentrations of DMSO and ethanol used as solvents in the cell culture medium were less than 0.1%. IPEC-J2 cells for subculture use were cultured in DMEM/F12 added with 10% fetal bovine serum (Biological Industries, Kibbutz Beit-Haemek, Israel) without antibiotics. The cells were routinely seeded at a density of 5 × 10⁵ in plastic tissue culture flasks (25 cm²), kept in a humidified incubator at 37 °C with 5% CO₂, and passaged twice weekly.

Huang W., Chang J., Wang P., Liu C., Yin Q., Song A., Gao T., Dang X, & Lu F. (2019). Effect of Compound Probiotics and Mycotoxin Degradation Enzymes on Alleviating Cytotoxicity of Swine Jejunal Epithelial Cells Induced by Aflatoxin B1 and Zearalenone. Toxins, 11(1), 12.

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Osteogenic Differentiation Protocol

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Calcium nitrate dihydrate (Ca(NO₃)₂.2H₂O), diammonium phosphate ((NH₄)₂HPO₄), boric acid (H₃BO₃),
ammonia (NH₄OH), acetic acid (glacial), l-ascorbic
acid, β-glycerophosphate,
and dexamethasone were purchased from Sigma, USA. DMEM:F12, fetal
bovine serum, penicillin–streptomycin cocktail, RPMI 1640,
EndoGo XF, and EndoGoXF supplements were purchased from Biological
Industries, Israel. PMA and Normocin were obtained from Invivogen,
USA. Other chemicals and solutions used in the study are reagent-grade.

Pazarçeviren A.E., Akbaba S., Evis Z, & Tezcaner A. (2022). Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and Regeneration. ACS Biomaterials Science & Engineering, 8(7), 3038-3053.

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HT-29 Cells Maintenance Protocol

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Human colon carcinoma cells (HT-29 cells) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China), which were respectively maintained in Dulbecco’s Modified Eagle Medium/F12 medium (DMEM/F12) supplemented with 10% (v/v) fetal bovine serum (FBS, Biological Industries, Kibbutz Beit-Haemek, Israel) under a humidified atmosphere of 5% CO₂ humidified air at 37°C.

Tong X., Liang R., Jia Y., Qin W., Guo C., Wu X., Wang Z., Chen D, & Tan N. (2019). Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion. Frontiers in Pharmacology, 10, 1422.

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Adipocyte Differentiation Protocol

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Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).

Liang C., Gao S., Gao J., Xu Y, & Li Q. (2023). Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH. Scientific Reports, 13, 18431.

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Chondrocyte Isolation and L-Theanine Treatment

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Primary chondrocytes were isolated from 14- to 21-day-old rats. After trypsinization (0.25%, GIBCO, New York, USA) of cartilage for 30 min, the slice was treated with 0.2% collagenase II for 4 h at 37 °C. The culture medium was then cultured with DMEM/F12, including 10% fetal calf serum (Biological Industries, Israel). The steps were consistent with previous research [24 (link)] and the second passage cells were chosen for the subsequent experiments. Identification of chondrocytes was stained with toluidine blue for proteoglycans and immunohistochemical staining for type II collagen (Novus Biologicals, CO, USA).
The medium was cultured with DMEM/F12 containing 0.5% serum starving for 12 h and were stimulated with IL-1β (PeproTech Inc. USA) for 24 h prior to being co-cultured with different concentrations of L-theanine (50, 100, 200 μM) for 24 h. Cells treated with IL-1β only served as the control. The dose of L-theanine (≥98% (high-performance liquid chromatography, HPLC), Sigma-Aldrich, St. Louis, MO, USA) was determined according to previous studies which L-theanine showed inhibition of NF-κB [25 (link)] and anti-inflammation activities [26 (link)].

Bai H., Zhang Z., Li Y., Song X., Ma T., Liu C., Liu L., Yuan R., Wang X, & Gao L. (2020). L-Theanine Reduced the Development of Knee Osteoarthritis in Rats via Its Anti-Inflammation and Anti-Matrix Degradation Actions: In Vivo and In Vitro Study. Nutrients, 12(7), 1988.

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Primary Mammary Epithelial Cell Culture

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DMEM/F12, penicillin, streptomycin, amphotericin B, L-glutamine solution, trypsin—EDTA solution C and fetal bovine serum (FBS) were all obtained from Biological Industries (Beit Haemek, Israel). Bovine insulin, hydrocortisone, ovine prolactin, BSA solution, hyaluronidase and DNase I were purchased from Sigma Aldrich Israel Ltd. (Rehovot, Israel). Collagenase type II was purchased from Worthington Biochemical Corporation (Lakewood, NJ).

Cohen B.C., Shamay A, & Argov-Argaman N. (2015). Regulation of Lipid Droplet Size in Mammary Epithelial Cells by Remodeling of Membrane Lipid Composition—A Potential Mechanism. PLoS ONE, 10(3), e0121645.

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Cultivating Human iPSCs from Omental Stromal Cells

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iPSCs were generated from omental stromal cells and were a kind gift from Dr. Rivka Ofir from Ben Gurion University. The undifferentiated cells were cultivated on 10 cm culture plates precoated with Matrigel™ (Corning, NY, USA) diluted to 250 µg·mL⁻¹ in DMEM/F12 (Biological Industries, Kibbutz Beit-Haemek, Israel). Cells were maintained in NutriStem™ (Biological Industries) medium containing 0.1% penicillin/streptomycin (Biological Industries) and cultured under a humidified atmosphere at 37 °C with 5% CO₂. The medium was refreshed daily, and cells were passaged at 70% confluence by treatment with 1 mL of ReLeSR™ (Stemcell Technologies, Vancouver, BC, Canada).

Shilo M., Baruch E.S., Wertheim L., Oved H., Shapira A, & Dvir T. (2023). Imageable AuNP-ECM Hydrogel Tissue Implants for Regenerative Medicine. Pharmaceutics, 15(4), 1298.

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Expansion of Pancreatic Islet Cells

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Isolated islet cell clusters were washed twice with DMEM/F-12 (Biological Industries), counted and mixed with Matrigel in glass centrifuge tubes. In all, 30,000–50,000 cells or 10–20 cell clusters were used per well of a pre-warmed 24-well plate (the volume of 50 µL). After Matrigel was solidified for 30 min at 37 °C, pancreatic islet expansion medium (PIEM) was added. PIEM consists of DMEM/F-12 (1% GlutaMax, 1% Penicillin-Streptomycin) plus 15% RSPO1 conditioned medium (home-made), 3 µM CHIR-99021 (BioGems), 1 µM A83-01 (Adooq Bioscience), 10 nM gastrin-1 (MedChemExpress), 1.25 mM N-acetylcysteine (Sigma), 10 µM Y-27632 (Adooq Bioscience), 10 µM FSK (TargetMol), 50 ng/mL Exendin-4 (ChinaPeptides), 50 µg/mL L-Ascorbic acid (Sigma), 250 nM 5-Iodotubercidin (Adooq Bioscience), 50 ng/mL FGF10 (PeproTech), 50 ng/mL EGF (PeproTech), 10 mM Nicotinamide (Sigma), B27 Supplement (minus Vitamin A) (Thermo). Cultures were kept at 37 °C, 5% CO₂ in a humidified incubator. During culturing, medium was refreshed every three days.

Lin J.Y., Cheng J., Du Y.Q., Pan W., Zhang Z., Wang J., An J., Yang F., Xu Y.F., Lin H., An W.T., Wang J., Yang Z., Chai R.J., Sha X.Y., Hu H.L., Sun J.P, & Yu X. (2020). In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals. Cell Discovery, 6, 20.

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