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Dimethyl 9 9 biacridinium dinitrate lucigenin

Manufactured by Merck Group
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Dimethyl-9,9'-biacridinium dinitrate (Lucigenin) is a chemical compound used as a fluorescent probe in various analytical and research applications. It is a crystalline solid with a molecular formula of C30H26N4O6. Lucigenin emits light upon oxidation, making it useful for detection and quantification of certain analytes in chemical and biological systems.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Phenylmethanesulfonyl fluoride, by Merck Group (1 mentions) Acrylamide bis solution, by Bio-Rad (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Methanol, by Merck Group (1 mentions) Mitosox, by Thermo Fisher Scientific (1 mentions) Isopropanol, by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Sodium dodecyl sulphate (sds), by Merck Group (1 mentions) Leupeptin, by Merck Group (1 mentions) Etoposide, by Merck Group (1 mentions) Doxorubicin, by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Temed, by Merck Group (1 mentions) Sodium fluoride, by Merck Group (1 mentions) Sodium orthovanadate, by Merck Group (1 mentions) Pepstatin, by Merck Group (1 mentions)

Close spelling variants of this product

Lucigenin (151 citations) N,N′-dimethyl-9,9′-biacridinium dinitrate (lucigenin), (2 citations)

2 protocols using dimethyl 9 9 biacridinium dinitrate lucigenin

1

Reagents and Chemicals for Cell Culture Studies

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Fetal Bovine Serum (FBS) and Phosphate Buffered Saline (PBS) were purchased from Hyclone™, ThermoFisher Scientific, Waltham, MA, USA. HEPES, EDTA, EGTA, TEMED, isopropanol, aprotonin, pepstatin, phenylmethanesulfonyl fluoride (PMSF), leupeptin, sodium fluoride (NaF), sodium orthovanadate (Na3VO4), diethyldithiocarbamate (DDC), doxorubicin, etoposide, fludarabine, 5-flouroUracil (5-FU), cisplatin, dimethyl-9,9’-biacridinium dinitrate (Lucigenin), N-acetyl-cysteine (NAC) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Venetoclax (VEN) was purchased from Medchem Express LLC. Streptomycin–penicillin and l-glutamine were purchased from Gibco. Coomassie Blue dye and bovine serum albumins (BSA) were purchased from ThermoFisher Scientific. Methanol and sodium dodecyl sulphate (SDS) were purchased from Merck, Kenilworth, NJ, USA. Sucrose and 30% acrylamide/bis solution were purchased from Bio-Rad. Goat anti-mouse and anti-rabbit IgG horseradish peroxidase conjugated secondary antibodies were purchased from Pierce, TX, USA. Protein A agarose beads were purchased from Santa Cruz, TX, USA. Chloromethyl-2-,7-dichlorofluorescin diacetate (DCFDA) and MitoSox™ were purchased from Molecular Probes, Thermo Fisher Scientific.
Chong S.J., Iskandar K., Lai J.X., Qu J., Raman D., Valentin R., Herbaux C., Collins M., Low I.C., Loh T., Davids M, & Pervaiz S. (2020). Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism. Nucleic Acids Research, 48(22), 12727-12745.
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2

Polymer-based Anti-Inflammatory Drug Delivery

Cited 1 time
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Poly (DL-lactic-co-glycolic) acid (85:15, ester-terminated, 0.55 – 0.75 dL/g) was from Durect Corporation (Birmingham, AL). PEG2000-hydrazone-C18 (PHC), PEG2000-amide-C18 (PAC), and O-stearoyl mannose (M-C18) were synthesized using methods previously published by our laboratory 20 (link). BA and prednisolone were from TCI America (Portland, OR). Cyanine7.5 NHS ester (Cy7.5) was from Lumiprobe (Hallandale Beach, FL). TNF-α ELISA kit was from Thermo-Fisher Scientific (Waltham, MA). LPS and dimethyl-9,9′-biacridinium dinitrate (lucigenin) were from Sigma-Aldrich (St. Louis, MO). All solvents were of HPLC grade and from Sigma Aldrich.
Mouse J774A.1 macrophage cells were from the American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% v/v fetal bovine serum (FBS) and penicillin/streptomycin (100 U/mL), all from Gibco (Grand Island, NY).
All female C57BL/6 mice (18–20 grams) were from Charles River Laboratories (Wilmington, MA). Animal studies were conducted following the U.S. National Research Council Guidelines for Care and Use of Laboratory Animals. The animal protocol was approved by the Institutional Animal Care and Use Committee at The University of Texas at Austin.
O’Mary H.L., Aldayel A.M., Valdes S.A., Naguib Y.W., Li X., Salvady K, & Cui Z. (2017). Acid-sensitive sheddable PEGylated, mannose-modified nanoparticles increase the delivery of betamethasone to chronic inflammation sites in a mouse model. Molecular pharmaceutics, 14(6), 1929-1937.
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