Zetasizer nano model zen3600

The zeta potential (ζ) and aggregate size of the chitin nanofiber hydrocolloid was studied by dynamic light scattering using Zetasizer Nano, Model ZEN3600 (Malvern Instruments Ltd., UK). The light source was operated at a wavelength of 633 nm. The chitin nanofiber suspension was diluted to a concentration of 50 mg/L at pH 3 and filled in a PMMA (Poly Methyl Methacrylate) cuvette and scanned three times at ambient conditions (i.e., 25°C).

The size and ζ-potential were measured by dynamic light scattering using a Zetasizer Nano model ZEN3600 (Malvern Panalytical, Malvern, UK). All measurements were performed in triplicate. Three different batches of each system were evaluated. For the size measurements, the samples were diluted 20-fold with distilled water and, for ζ-potential, 10 mM of sodium chloride solution was used at the same dilution. High resolution scanning electron microscopy images were acquired using an Extra-High Resolution Scanning Electron Microscope Magellan 400L (Thermo Fisher Scientific, Waltham, MA, USA, former FEI), equipped with a tunable diode laser (TLD) detector. The 10 µL samples were diluted 20-fold with water, as above, and placed on copper grid support films, formvar/carbon, 400 mesh, Cu (Ted Pella, Inc., Redding, CA, USA). The images were scanned at 5 kV acceleration voltages.