Dulbecco s modified eagle medium dmem

Silver nitrate and acetone were purchased from Sigma Aldrich, USA. Dulbecco's Modified Eagle Medium (DMEM) was obtained from Capricorn Scientific, Germany. Ethidium bromide (EB), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), were obtained from SERVA, Germany. Acridine Orange (AO) was obtained from Acros Organics, New Jersey, USA. Phosphate-buffered saline (PBS) without calcium and magnesium, normal melting point agarose (NMA), low melting point agarose (LMA), and collagenase were obtained from Alfa Trade Enterprise D.O.O.; ethyl methanesulphonate (EMS) were from Sigma-Aldrich, St. Louis, MO, USA. Resazurin was obtained from Alfa Aesar GmbH & Co., Karlsruhe, Germany. The chemicals used in this study for DPPH and ABTS radical scavenging assay: 2,2-diphenyl-1-picrylhydrazyl 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), referent phenolic standards (nordihydroguaiaretic acid (NDGA), (S)-6-methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox)), and methanol were procured from Sigma-Aldrich Chemicals. Tetracycline, ampicillin, and amphotericin B were obtained from Sigma Chemicals Co., USA, and itraconazole from Pfizer Inc., USA. All other solvents and chemicals were of analytical grade. In distilled water, all solutions were made.

A mixture of AAI and II (50% of each compound) was purchased from Acros Organics Co. (Geel, Belgium). Dulbecco’s Modified Eagle Medium (DMEM), penicillin/streptomycin, L-glutamine and accutase were obtained from Capricorn Scientific (Ebsdorfergrund, Germany). Fetal bovine serum (FBS) was purchased from Life Technologies Europe (Gent, Belgium), and sodium citrate, from Merck (Darmstadt, Germany). Resazurin, 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) and nebivolol hydrochloride were obtained from Sigma (St. Louis, MO, USA).

Frozen blueberry (Vaccinium myrtillus L.) peels were provided by Indena S.p.a. (Settala, Milano, Italy) as industrial residues. The matrix was stored at −18 °C.
The choline chloride, malic acid, citric acid, lactic acid, glycerol, and glucose used for NADES preparation were ACS grade ≥90%. Ethanol (ACS grade, ≥99%) and hydrochloric acid (37%) were used for anthocyanin extraction, purification, and NADES recycling. Sodium bisulfite (ACS grade, ≥99%) was used to determine total anthocyanin content, while potassium metabisulfite (ACS grade, ≥99%) was used for percentage polymeric colour evaluation. All the above-mentioned chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The two adherent human cell lines used (HeLa and HaCaT) were purchased from ATCC (Manassas, VA, USA). The Dulbecco’s modified Eagle medium (DMEM) and foetal bovine serum (FBS) used for cell cultivation were purchased from Capricorn (Ebsdorfergrund, Germany). Antibiotic/antimitotic solutions and trypsin-EDTA solutions (0.25%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The CellTiter 96^® AQ_ueous One Solution Cell Proliferation assay was purchased from Promega (Madison, WI, USA). The Muse™ Annexin V & Dead Cell Kit was purchased from Merck KGaA (Darmstadt, Germany).

Avermectin B1a was purchased from BioAustralis (BIA-A1010, BioAustralis, New South Wales, Australia), paclitaxel was obtained from Cytoskeleton (TXD01, Cytoskeleton, Denver, CO, USA), and colchicine was provided by Sigma Aldrich (C9754, Sigma Aldrich, St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM), the buffer Dulbecco’s PBS, and L-Glutamine (200 mM) were purchased from Capricorn (Capricorn, Dusseldorf, Germany), while fetal bovine serum (FBS), penicillin/streptomycin, and trypsin/EDTA were obtained from Biological Industries (Biological Industries, Jezreel Valley, Israel). The cell-counting dye trypan blue (0.4%) was purchased from Thermo Fisher Scientific (15250-061, Thermo Fisher Scientific, Waltham, MA, USA). Dimethyl sulfoxide (DMSO) was obtained from Carlo Erba (445106, Carlo Erba, Val de Reuil, Normandie, France).
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used for cell viability assay and was purchased from Thermo Fisher Scientific (M6494, Thermo Fisher Scientific, USA). A tubulin polymerization assay was performed by using a tubulin polymerization assay kit from Cytoskeleton (BK004P, Cytoskeleton, Denver, CO, USA), whereas the apoptosis assay was performed by using annexin V-FITC/PI apoptosis kit purchased from Elabscience (E-CK-A211, Elabscience, Huston, TX, USA).