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Anti cd77

Manufactured by Bio-Rad
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Anti-CD77 is a monoclonal antibody that binds to the CD77 antigen, also known as the globoside antigen. CD77 is a glycosphingolipid expressed on the surface of some cell types, including B cells and certain tumor cells. The primary function of Anti-CD77 is to detect and identify cells expressing the CD77 antigen.

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Lab products found in correlation

Automacs system, by Miltenyi Biotec (2 mentions) Anti mouse igg1 microbeads, by Miltenyi Biotec (2 mentions) Anti fitc microbeads, by Miltenyi Biotec (2 mentions) Anti igd fitc, by BD (2 mentions) Mca579, by BD (1 mentions)

2 protocols using anti cd77

1

Isolation and Characterization of Naive and Germinal Center B Cells

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Tonsils were minced, and mononuclear cells were isolated using Ficoll Histopaque density centrifugation. Naïve B cells were separated by positive selection using AutoMACS system (Miltenyi Biotec) after incubation with anti-IgD-FITC (BD Pharmingen) followed by anti-FITC microbeads (Miltenyi Biotec). Germinal center B cells (GCB) were separated by positive selection with anti-CD77 (AbD Serotec) followed by mouse anti-IgM, IgG1 isotype (BD Pharmingen) and anti-mouse-IgG1 microbeads (Miltenyi Biotec). Naive and germinal center B-cell purity (>90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences, 555778), CD77 (Bio-Rad, MCA579) and CD38 (BD Biosciences, 340439). GCB were further verified by expression of A4GALT and BCL6 by RT-qPCR.
Calvo-Vidal M.N., Zamponi N., Krumsiek J., Stockslager M.A., Revuelta M.V., Phillip J.M., Marullo R., Tikhonova E., Kotlov N., Patel J., Yang S.N., Yang L., Taldone T., Thieblemont C., Leonard J.P., Martin P., Inghirami G., Chiosis G., Manalis S.R, & Cerchietti L. (2021). Oncogenic HSP90 Facilitates Metabolic Alterations in Aggressive B-cell Lymphomas. Cancer Research, 81(20), 5202-5216.
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2

Isolation of Naive and Germinal Center B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tonsils were minced, and mononuclear cells were isolated using Ficoll Histopaque density centrifugation. Naïve B cells were separated by positive selection using AutoMACS system (Miltenyi Biotec) after incubation with anti-IgD-FITC (BD Pharmingen) followed by anti-FITC microbeads (Miltenyi Biotec). Germinal center B cells (GCB) were separated by positive selection with anti-CD77 (AbD Serotec) followed by mouse anti-IgM, IgG1 isotype (BD Pharmingen) and anti-mouse-IgG1 microbeads (Miltenyi Biotec). Naive and germinal center B cell purity (> 90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579) and CD38 (BD Biosciences 340439). GCB were further verified by expression of A4GALT and BCL6 by RT-qPCR.
Calvo-Vidal M.N., Zamponi N., Krumsiek J., Stockslager M.A., Revuelta M.V., Phillip J.M., Marullo R., Tikhonova E., Kotlov N., Patel J., Yang S.N., Yang L., Taldone T., Thieblemont C., Leonard J.P., Martin P., Inghirami G., Chiosis G., Manalis S.R, & Cerchietti L. (2021). Oncogenic HSP90 Facilitates Metabolic Alterations in Aggressive B-cell Lymphomas. Cancer Research, 81(20), 5202-5216.
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