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Phalloidin alex647

Manufactured by Thermo Fisher Scientific
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Phalloidin Alex647 is a fluorescent dye used for labeling and visualizing actin filaments in cells. It binds specifically to F-actin and emits fluorescence in the far-red/near-infrared spectrum.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (2 mentions) Human fibronectin, by Thermo Fisher Scientific (2 mentions) Biotinylated ecl, by Vector Laboratories (2 mentions) Dihydrorhodamine 123 dhr 123, by Thermo Fisher Scientific (1 mentions) Hrp conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Hrp conjugated anti flag antibody, by Merck Group (1 mentions) Ripa buffer, by Merck Group (1 mentions) Mouse anti flag m2 antibody, by Merck Group (1 mentions) Pngase f, by New England Biolabs (1 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions) N formyl met leu phe fmlp, by Merck Group (1 mentions)

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Phalloidins (2 citations)

2 protocols using phalloidin alex647

1

Characterization of Leukocyte Adhesion

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Mouse anti-FLAG M2 antibody (Sigma), HRP-conjugated anti-FLAG antibody (Sigma), APC PSGL-1 antibody (FLEG) (ThermoFisher), Human Fibronectin (ThermoFisher), DMSO (Sigma), Phalloidin Alex647 (ThermoFisher), RIPA buffer (Sigma), Phorbol 12-Myristate 13-Acetate (PMA, sigma), SLeX (Sigma), Biotinylated ECL (Vector Laboratories), PNGase F (New England Biolabs), Dihydrorhodamine 123 (DHR123) (ThermoFisher), ProLong Gold Antifade Mountant (ThermoFisher), HRP-conjugated streptavidin (ThermoFisher).
Chen C., Yang C, & Barbieri J.T. (2019). Staphylococcal Superantigen-like protein 11 mediates neutrophil adhesion and motility arrest, a unique bacterial toxin action. Scientific Reports, 9, 4211.
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2

Fibronectin Extracellular Matrix Assay

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Reagents were human fibronectin (ThermoFisher), DMSO (Sigma), Phalloidin Alex647 (ThermoFisher), N-formyl-Met-Leu-Phe (fMLP, sigma), SLeX (Sigma), biotinylated ECL (Vector Laboratories), ProLong Gold Antifade Mountant (ThermoFisher), and Q5 Site-Directed Mutagenesis Kit (New England BioLabs).
Yang C., Barbieri J.T., Dahms N.M, & Chen C. (2022). Multiple Domains of Staphylococcal Superantigen-like Protein 11 (SSL11) Contribute to Neutrophil Inhibition. Biochemistry, 61(7), 616-624.
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