All chemicals were obtained from Sigma (St. Louis, MO) unless otherwise stated. ZBD-2 was synthesized at our Laboratory with the purity of 99.9% and dissolved in the saline (0.9% NaCl). Rabbit anti-NR2A, mouse anti-NR2B, and mouse anti-GAD67 antibodies were purchased from Millipore (Billerica, MA). Mouse anti-CaMKIIα antibody was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA). Rabbit anti-GluR1 antibody was purchased from Abcam (Cambridge, UK). All of the chemicals and reagents used were commercially available and of standard biochemical quality.
Rabbit anti glur1 antibody
Manufactured by Abcam
Sourced in United Kingdom, United States
Rabbit anti-GluR1 antibody is a primary antibody that binds to the GluR1 subunit of the AMPA-type glutamate receptor. It can be used for the detection and analysis of the GluR1 protein using techniques such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti glur1 antibody
1
Biochemical Markers of Neural Activity
2
Spinal Cord Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After being deeply anaesthetized, the L4–L6 spinal cord was removed and transferred
quickly to ice-chilled radio-immunoprecipitation assay (RIPA) lysis buffer. The
homogenates were centrifuged at 10,000× g for 15 min at 4°C to separate the total protein
from the precipitate. A membrane compartment protein extraction kit (BioChain Institute,
Inc., USA) was used to extract the membrane fraction of the dorsal horn. The concentration
of the total protein was measured by the BCA protein assay kit. The following primary
antibodies were used: rabbit anti- P-Rex2 antibody (1:1000; Sigma), rabbit anti- Rac1
antibody (1:1000, Abcam), rabbit anti-phospho Rac1 plus Cdc42 (1:200, Santa Cruz, USA),
rabbit anti- GluR1 antibody (1:1000, Abcam), and rabbit anti-pGluR1 antibody (1:1000,
Abcam). Western blot assay was performed as previously described.11 (link)
quickly to ice-chilled radio-immunoprecipitation assay (RIPA) lysis buffer. The
homogenates were centrifuged at 10,000× g for 15 min at 4°C to separate the total protein
from the precipitate. A membrane compartment protein extraction kit (BioChain Institute,
Inc., USA) was used to extract the membrane fraction of the dorsal horn. The concentration
of the total protein was measured by the BCA protein assay kit. The following primary
antibodies were used: rabbit anti- P-Rex2 antibody (1:1000; Sigma), rabbit anti- Rac1
antibody (1:1000, Abcam), rabbit anti-phospho Rac1 plus Cdc42 (1:200, Santa Cruz, USA),
rabbit anti- GluR1 antibody (1:1000, Abcam), and rabbit anti-pGluR1 antibody (1:1000,
Abcam). Western blot assay was performed as previously described.11 (link)
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