The human gallbladder carcinoma cell line NOZ was originally obtained from Health Science Research Resources Bank (HSRRB) in Japan. Human dermal lymphatic endothelial cells (HDLECs) was from Sciencell (San Diego, California, USA). NOZ cell line was maintained in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Life Technologies Gibco, Carlsbad, California). HDLECs were incubated in endothelial cell medium (ECM, Sciencell). Both of the cell lines were incubated at 37°C in a 5% CO2 humidified incubator.
Hdlec
Manufactured by ScienCell
Sourced in United States
HDLECs are primary human dermal lymphatic endothelial cells. They are characterized by the expression of lymphatic-specific markers and the ability to form tube-like structures in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Endothelial cell medium (ecm), by ScienCell (8 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Cell counting kit 8 solution, by Dojindo Laboratories (1 mentions)
Panc 1, by Cytiva (1 mentions)
Rpmi 1640 hyclone, by Cytiva (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Sw1990, by Merck Group (1 mentions)
Caski, by American Type Culture Collection (1 mentions)
Me 180, by American Type Culture Collection (1 mentions)
Ms751, by American Type Culture Collection (1 mentions)
Qbc939, by Thermo Fisher Scientific (1 mentions)
Qbc939, by National Collection of Authenticated Cell Cultures (1 mentions)
Endothelial cell growth factor, by ScienCell (1 mentions)
Endothelial cell growth medium, by ScienCell (1 mentions)
Dulbecco minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Qbc939, by ScienCell (1 mentions)
Hebepics, by ScienCell (1 mentions)
20 protocols using hdlec
1
NOZ and HDLEC Cell Culture
2
Endothelial Cell Culture and Stimulation
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HdLECs were purchased from ScienCell (Carlsbad, CA). HdLECs were cultured at 37 C with 5% CO 2 in endothelial cell medium (ScienCell) supplemented with 20% fetal bovine serum, 100 U/mL penicillin, 100 ng/mL streptomycin, and 100 mg/mL endothelial cell growth supplement (ScienCell). HdLECs were treated with 1 mg/mL LPS (Sigma-Aldrich, St. Louis, MO), recombinant human LTa 3 (R&D Systems, Minneapolis, MN) and 100 ng/mL LTa 1 b 2 (R&D Systems), or 100 ng/mL recombinant human LIGHT (Peprotech, Rocky Hill, NJ) in endothelial cell medium containing 2% fetal bovine serum and further cultured for 24 hours.
3
HMGB1 Modulates HDLEC Proliferation
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Human dermal lymphatic endothelial cells (HDLECs) were purchased from ScienCell (Carlsbad, CA) and maintained in endothelial cell basal medium-2 with growth supplements (EBM-2 MV). For the proliferation assay, HDLECs were pre-incubated with different doses of HMGB1 (0 ng/mL, 50 ng/mL, 100 ng/mL, 500 ng/mL, 1000 ng/mL or 2000 ng/mL) for 1 hour, followed by incubation with or without VEGF-C (10 ng/mL). The cells were allowed to proliferate for 24 hours, and 100 μL of cells from each well was transferred to a new 96-well plate with 10 μL of Cell Counting Kit-8 solution (Dojindo Laboratories, Kumamoto, Japan). The absorbance at 450 nm was then measured using a microplate reader.
The RAW264.7 murine macrophage cell line were purchased from Shanghai Cell Bank of Academia Sinica (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium supplemented with 2 mM L-glutamine and 10% heat-inactivated foetal bovine serum (FBS).
The RAW264.7 murine macrophage cell line were purchased from Shanghai Cell Bank of Academia Sinica (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium supplemented with 2 mM L-glutamine and 10% heat-inactivated foetal bovine serum (FBS).
4
Lentiviral Transduction of Cancer and Endothelial Cells
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The human cervical cancer cell line SiHa was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured according to the supplier’s guidelines. HDLECs were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured in endothelial cell medium (ECM; ScienCell) with 5% FBS (Gibco, Invitrogen, Carlsbad, CA, USA). SiHa cells were transfected with lenti-mCherry; cells stably expressing mCherry were selected for further experiments. HDLECs were transfected with lenti-GFP, and cells stably expressing GFP were selected.
5
Culturing Human Dermal Lymphatic Endothelial Cells
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Human dermal lymphatic endothelial cells (HDLECs) were purchased from ScienCell (Carlsbad, CA, USA) and grown in endothelial cell basal medium-2 with growth supplements (EBM-2 MV) at 37 °C in a humidified 95%/5% (vol/vol) air/CO2 atmosphere.
6
Culturing Pancreatic and Endothelial Cells
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The PC cell lines PANC-1 and SW1990, immortalized human pancreatic ductal epithelial cells (HPDCs) and human lymphatic tube endothelial cells (HDLECs) were purchased from Guangzhou Genio Biotech Co., Ltd. The SW1990 and HPDC cells were cultured in DMEM (MilliporeSigma), the PANC-1 cells were cultured in RPMI-1640 (HyClone; Cytiva) and the HDLECs were cultured in Endothelial Cell Medium (ScienCell Research Laboratories, Inc.), each supplemented with 10% FBS (Thermo Fisher Scientific, Inc.) and 1% antibiotics (penicillin-streptomycin; Thermo Fisher Scientific, Inc.). Culture was performed under normoxic or hypoxic conditions in a humidified incubator at 37°C. The normoxic conditions were 20% O2, 5% CO2 and 75% N2, and the hypoxic conditions were 1% O2, 5% CO2 and 94% N2. Cells in the logarithmic growth stage were selected for subsequent experiments.
7
Cell Line Culture Protocol
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The human CCa cell lines Siha, Caski, HeLa, C33A, ME180, and MS751 were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured according to the respective guidelines. hCEp cells (#7060) and HDLECs (#2010) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured in cervical epithelial cell medium (CerEpiCM; Cat. #7601; ScienCell) and endothelial cell medium (ECM; Cat. #1001; ScienCell), respectively, in a humidified incubator with 5% CO2 at 37°C. CCa cell lines were used within ten passages, whereas hCEp cells and HDLECs were used within six passages.
8
Cell Culture of QBC939 and HDLECs
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CCA cell line QBC939 was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human dermal lymphatic endothelial cells (HDLECs) were purchased from Scien Cell Research Laboratories (Carlsbad, CA, USA). QBC939 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin/streptomycin. HDLECs were cultured in endothelial cell medium with 1% endothelial cell growth supplement, 5% FBS and 1% penicillin/streptomycin. HDLECs which were passaged between 2 and 7 times were used for later experiments. Cells were grown at 37°C in a humidified incubator with 5% CO2.
9
Culturing Colon Cancer and Lymphatic Cells
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Human colonic adenocarcinoma HT-29 cell lines were provided by the Institute of Cell and Biochemistry, Chinese Academy of Sciences (Shanghai, China), and grown in RPMI-1640 medium supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) in an incubator (Forma Scientific, USA) at 37 °C under a mixture of 95 % air and 5 % CO2.
Human lymphatic endothelial cells were primary HDLECs purchased from ScienCell Research Laboratories, USA. Cells were identified by immunefluorescent cytochemical technique via CD31, Podoplanin and LYVE-1, and grown in endothelial cell growth medium (ECGM) with endothelial cell growth factor (ScienCell Research Laboratories) in an incubator (Forma Scientific) with 5 % CO2 at 37 °C as described previously [23 ], then were used in the experiments at fifth generation of the cells.
Human lymphatic endothelial cells were primary HDLECs purchased from ScienCell Research Laboratories, USA. Cells were identified by immunefluorescent cytochemical technique via CD31, Podoplanin and LYVE-1, and grown in endothelial cell growth medium (ECGM) with endothelial cell growth factor (ScienCell Research Laboratories) in an incubator (Forma Scientific) with 5 % CO2 at 37 °C as described previously [23 ], then were used in the experiments at fifth generation of the cells.
10
Cervical Cancer Cell Line and Endothelial Cell Transfection
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The human cervical cancer cell line, SiHa., was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured according to the supplier's guidelines. Human dermal lymphatic endothelial cells (HDLECs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured in endothelial cell medium (ECM; ScienCell) with 5% FBS (Gibco, Invitrogen, Carlsbad, CA, USA). The SiHa cells were transfected with lenti‐mCherry; cells stably expressing mCherry fluorescent protein signals were selected for further experiments. On the other hand, the HDLECs were transfected with lenti‐GFP to stably express GFP fluorescent protein signals.
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