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Chicken anti gfp a10262

Manufactured by Thermo Fisher Scientific

Chicken anti-GFP (A10262) is a primary antibody that specifically binds to the green fluorescent protein (GFP). It is designed for use in a variety of immunodetection applications, such as Western blotting, immunohistochemistry, and flow cytometry.

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4 protocols using chicken anti gfp a10262

1

Comprehensive Immunolabeling Panel for Neuroscience

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The rabbit anti-ANK3 (Ankyrin-G; sc-28561), the goat anti-SOX1 (sc-17318) and the HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). The chicken anti-GFP (A10262) and rat anti-mCherry (M11217) were from Life Technologies. The mouse anti-PSD95 (Clone K28/43, 75-028) was from NeuroMab, the rabbit anti-SOX2 (ab59776) from Abcam (Cambridge, MA), and the rabbit anti-Tuj1 (PRB-435P) from Covance (Richmond, CA). The rabbit anti-CACNB3 was purchased from LifeSpan BioSciences (Seattle, WA). The mouse monoclonals anti-Synaptophysin (S5768) and the anti-β-actin (A1978) were from Sigma. Finally, the rabbit anti-Musashi (AB5977) was from Millipore and the mouse anti-Synapsin 1 (106 001) as well as guinea pig anti-MAP2 (188 004) were purchased from Synaptic Systems (Gottingen, Germany).
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2

Neuronal Marker Immunostaining Protocol

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Mouse anti-NeuN antibody (MAB377) was obtained from Millipore. Rabbit anti-VGLUT1 antibody (VGT1-3) was purchased from Mab Technologies. Chicken anti-GFP (A10262), donkey anti-mouse IgG AlexaFluor 594, anti-chicken AlexaFluor 488 and anti-rabbit IgG AlexaFluor 594 antibodies were purchased from Life Technologies. Other common reagents were from Sigma-Aldrich or Fisher Scientific.
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3

Comprehensive Immunolabeling Panel for Neuroscience

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The rabbit anti-ANK3 (Ankyrin-G; sc-28561), the goat anti-SOX1 (sc-17318) and the HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). The chicken anti-GFP (A10262) and rat anti-mCherry (M11217) were from Life Technologies. The mouse anti-PSD95 (Clone K28/43, 75-028) was from NeuroMab, the rabbit anti-SOX2 (ab59776) from Abcam (Cambridge, MA), and the rabbit anti-Tuj1 (PRB-435P) from Covance (Richmond, CA). The rabbit anti-CACNB3 was purchased from LifeSpan BioSciences (Seattle, WA). The mouse monoclonals anti-Synaptophysin (S5768) and the anti-β-actin (A1978) were from Sigma. Finally, the rabbit anti-Musashi (AB5977) was from Millipore and the mouse anti-Synapsin 1 (106 001) as well as guinea pig anti-MAP2 (188 004) were purchased from Synaptic Systems (Gottingen, Germany).
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4

Immunofluorescence Staining Methodology

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The following primary antibodies were used for cell culture and/or whole mount stainings: Rabbit anti-cleaved PARP Ab2317 [38 (link)] (1:500, Abcam), chicken anti-GFP A10262 [39 (link)] (1:400, Life Technologies), and mouse anti-MeCP2 ab55538 [40 (link)] (1:400, Abcam). Rabbit cleaved caspase-3 ASP175 [35 (link)] (1:100, Cell Signaling Technology) was used to confirm apoptotic nuclei (data not shown). All secondary antibodies, including goat anti-chicken Alexa 488 (Life Technologies A-11039), donkey anti-Mouse Alexa 568 (Life Technologies A10037), donkey anti-mouse Alexa 647 (Jackson Immuno Research 715-605-150), and donkey anti-rabbit Cy5 (Jackson ImmunoResearch 711-175-152) were applied at a concentration of 1:400.
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