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14 protocols using onetouch ultravue

1

Continuous Glucose Monitoring and Glycemic Metrics

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Interstitial glucose levels were monitored using the CGM System (iPro2®, Medtronic, Northridge, CA), which records glucose level every 5 min for up to 72 h. Sensor calibration was accomplished by self-monitored blood glucose values measured by OneTouch UltraVue (Johnson & Johnson, Tokyo, Japan). The mean blood glucose level, standard deviation (SD), mean amplitude of glycemic excursion (MAGE), percentage of time at blood glucose <70.2 mg/dl, and percentage of time at blood glucose ≥180 mg/dl were measured from the data recorded through CGM.
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2

Glucose Tolerance Test Protocol

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After fasting for 15 hours, a glucose solution was intraperitoneally injected (1 g/kg body weight). Blood was obtained from the tail vein before (0 minutes) and at 15, 30, 60, and 120 minutes after the injection. Glucose levels were measured by a ONETOUCH UltraVue (Johnson & Johnson, Tokyo, Japan).
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3

Circadian Monitoring of Glycemia

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Blood samples in each group were obtained from the caudal vein at the same time of day (10:00 am-11:00 am) once a week for 17 weeks of treatment in rats and once a week for 4 consecutive weeks of treatment in mice. Blood glucose was measured by using a blood glucose meter, ONETOUCH Ultra Vue (Johnson & Johnson Corp., New Brunswick, NJ, USA).
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4

Continuous Glucose Monitoring and Glycemic Metrics

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Blood glucose levels were measured for two consecutive days by CGM (CGMS-gold; Medtronic Minimed, Northridge, CA, USA) with SMBG using portable blood glucose meters (One Touch UltraVue; Johnson & Johnson, Tokyo, Japan). We evaluated the 8-hour glucose AUC using MIET and CGM. Moreover, we evaluated several CGM-related parameters [9 ]. Mean glucose levels, standard deviations (SDs) and coefficient of variation (CV) were calculated for all glucose data for 8 hours [9 ]. The mean amplitude of glucose excursion (MAGE) was calculated as the arithmetic mean of the differences between consecutive blood glucose peaks and nadirs provided that the differences are greater than one SD of the mean glucose value. M value was calculated with following formula: ∑ |10×log (blood glucose/100)|3/n, and the J index was calculated as follows: 0.001 (mean blood glucose+SD)2 [15 (link)16 (link)17 (link)].
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5

Glucose Tolerance Evaluation Protocol

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After overnight fasting for 16 h, FG levels were measured with glucometer (Onetouch UltraVue, Johnson & Johnson, USA) through the tail vein. IPGTT was performed by intraperitoneal injection of 2 g/kg glucose. Then blood glucose levels at 15, 30, 60, 90, and 120 min following glucose injection were measured via the tail vein.
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6

Continuous Glucose Monitoring During TPN

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The One Touch Ultra Vue glucometer (Johnson and Johnson, USA) was used to collect BG levels 6 times per day (interval survey 6 h during each time) during the whole TPN infusion. The average BG values within 24 h were considered BG levels for a giving day.
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7

Urinary Biomarkers for Glucose Homeostasis

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The level of blood glucose was determined using tail blood and a OneTouch UltraVue (Johnson & Johnson) or Antsense III (Horiba) glucometer. Urinary albumin was measured in the 24-h urine collections using an LBIS Mouse Albumin ELISA Kit according to the manufacturer's instructions (Fujifilm). The urine creatinine concentration of the 24-h urine was detected by using the LC‒MS/MS method, and urine osmolality was examined by a freezing point depression method. For assessment of fractional excretion of glucose, the urine glucose concentration was measured by using a Glucose Assay Kit-WST according to the manufacturer's instructions (Dojindo).
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8

Diabetes Induction in Mice

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STZ (WAKO, Tokyo, Japan) was injected into 6-week-old male mice for five days (50 mg/kg/day via intraperitoneal injection). Blood glucose was measured by OneTouch® UltraVue (Johnson and Johnson, New Brunswick, NJ, USA) three days after the STZ injection, and was defined as true diabetes, not prediabetes, if the serum glucose levels were higher than 250 mg/dl.
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9

Oral Glucose Tolerance Test in Mice

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Mice were fasted for 6 h before the OGTT. Fasting glucose levels were measured using a OneTouch UltraVue glucometer (Johnson & Johnson, New Brunswick, NJ, USA) and LFS Quick Sensor (Johnson & Johnson). The fasted mice were orally gavaged with a 20% (w/v) glucose solution (2 g/kg body weight (BW)). Thereafter, blood glucose levels were measured at 15, 30, 60, and 120 min.
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10

Continuous Glucose Monitoring in Acute Coronary Syndrome

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All patients underwent continuous glucose monitoring (CGM) for 48 h during the subacute period (7–10 days after emergent PCI and admission) using a CGMS (iPro2; Medtronic, Minneapolis, Minnesota, USA). The CGMS sensor was inserted under the skin to measure glucose levels in tissue fluid. We calibrated the CGMS with finger sticks four times per day (Onetouch Ultravue; Johnson & Johnson, New Brunswick, New Jersey, USA) to ensure optimal accuracy of the glucose sensor. Energy-controlled meals were served (25–28 kcal/kg of ideal body weight). The recorded 48-h CGM data were independently analyzed by two cardiologists who were blinded to the MBG and STRes data of each patient (Fig. 1). The SD, mean, minimum, and maximum PG during CGM were recorded. Time in hypoglycemia was defined as the time when PG levels were less than 70 mg/dl. The MAGE was calculated as the average value of the blood glucose fluctuation range that exceeded 1 SD of the mean glucose 15 (link).
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