Bc0305

Manufactured by Solarbio

Sourced in China

The BC0305 is a piece of lab equipment. It is used for conducting experiments or analyses in a laboratory setting. The core function of this product is to facilitate certain laboratory procedures, but a detailed description cannot be provided without the risk of bias or extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Bc0345, by Solarbio (3 mentions) Bc0595, by Solarbio (2 mentions) Bc0625, by Solarbio (2 mentions) Bc0685, by Solarbio (2 mentions) Bc0955, by Solarbio (2 mentions) Bc0730, by Solarbio (1 mentions) Bc0980, by Solarbio (1 mentions) Bc2235, by Solarbio (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Bc1175, by Solarbio (1 mentions) Bc0175, by Solarbio (1 mentions) Bc0025, by Solarbio (1 mentions)

7 protocols using bc0305

Skeletal Muscle Metabolite and Enzyme Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Content of adenosine triphosphate (ATP), triglyceride (TG), free fatty acid (FFA), glycogen, and acetyl-CoA as well as enzyme activity of lactic dehydrogenase (LDH), succinate dehydrogenase (SDH), acetyl-CoA carboxylase (ACC) and pyruvate carboxylase (PC) in skeletal muscle were measured using commercially available kits (BC0305, BC0625, BC0595, BC0345, BC0980, BC0685, BC0955, BC0410 and BC0730, Solarbio, China) according to the manufacturer’s instructions.

Cai B., Ma M., Zhang J., Kong S., Zhou Z., Li Z., Abdalla B.A., Xu H., Zhang X., Lawal R.A, & Nie Q. (2022). Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. Cell Death & Disease, 13(4), 389.

+ Open protocol

+ Expand

Measuring Metabolic Biomarkers in Incubation

Check if the same lab product or an alternative is used in the 5 most similar protocols

pH value of the incubation model was measured using a portable pH meter (FE20, Switzerland). The pH meter probe was inserted into the buffer, and three technical replicates of each sample were measured. The levels of glycogen, lactate, and ATP were analyzed using assay kits (BC0345, BC2235, BC0305, Solarbio, China) according to their specifications.

Huang C., Zhang D., Blecker C., Zhao Y., Xiang C., Wang Z., Li S, & Chen L. (2024). Effects of phosphoglycerate kinase 1 and pyruvate kinase M2 on metabolism and physiochemical changes in postmortem muscle. Food Chemistry: X, 21, 101125.

+ Open protocol

+ Expand

Skeletal Muscle Metabolite Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Content of TG, FFA, ATP, and glycogen as well as enzyme activity of LDH and SDH in skeletal muscle were measured using commercially available kits (BC0625, BC0595, BC0305, BC0345, BC0685, BC0955, respectively; Solarbio, China), according to the manufacturer's instructions.

Cai B., Ma M., Zhang J., Wang Z., Kong S., Zhou Z., Lian L., Zhang J., Li J., Wang Y., Li H., Zhang X, & Nie Q. (2021). LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2. Molecular Therapy. Nucleic Acids, 27, 319-334.

+ Open protocol

+ Expand

Midgut ATP Content after 20E Injection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sixth-instar larvae at 2 h after molting were selected. Six larvae were used in each treatment. Larvae were injected with 5 μl 20E (1.2 μg, 1 μM) or 5 μl DMSO (diluted with 1× PBS to 1:10,000, the control group), and the midguts were excised at 24 h after injection.
Above collected-midguts were grounded and used for ATP content detection by the ATP content detection kit (BC0305) (Solarbio) and the microplate reader (BioTek). At least three biological replicates were used.

Chang Y., Zhang B., Du M., Geng Z., Wei J., Guan R., An S, & Zhao W. (2022). The vital hormone 20-hydroxyecdysone controls ATP production by upregulating binding of trehalase 1 with ATP synthase subunit α in Helicoverpa armigera. The Journal of Biological Chemistry, 298(2), 101565.

+ Open protocol

+ Expand

Quantifying ATP Levels in B16-F10 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ATP levels in CPP-IL-12-treated B16-F10 cells was detected using ATP production was detected by using ATP assay kit in accordance with the manufacturer’s instructions (BC0305, Solarbio, Beijing, China).

Shen H.H., Peng J.F., Wang R.R., Wang P.Y., Zhang J.X., Sun H.F., Liang Y., Li Y.M., Xue J.N., Li Y.J., Sun G.B, & Xie S.Y. (2024). IL-12-Overexpressed Nanoparticles Suppress the Proliferation of Melanoma Through Inducing ICD and Activating DC, CD8+ T, and CD4+ T Cells. International Journal of Nanomedicine, 19, 2755-2772.

+ Open protocol

+ Expand

Quantifying ATP Levels in RSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

ATP levels in RSCs were tested using an ATP content assay kit (microassay, BC0305, Solarbio). The energy charge is the main parameter to describe the state of energy metabolism of cells. Detecting ATP content and calculating energy charges can reflect the state of energy metabolism. All testing procedures were performed according to the kit instructions.

Dong X., Zhang H., Duan P., Liu K., Yu Y., Wei W., Wang W., Liu Y., Cheng Q., Liang X., Huo Y., Yan L., Yu A, & Dai H. (2023). An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment. Science Advances, 9(51), eadi1078.

+ Open protocol

+ Expand

Oxidative Stress Biomarker Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercial kits were used to measure SOD activity (BC0175, Solarbio, China), as well as concentration of MDA (BC0025, Solarbio, China), GSH (BC1175, Solarbio, China), and ATP (BC0305, Solarbio, China). Briefly, fresh hippocampal samples were incubated in extraction solutions and completely homogenized on ice. Following centrifugation at 4°C for 10 minutes, the supernatants were collected to perform follow-up tests according to the manufacturer's instructions.

Hu X., Wu L., Wen Y., Liu J., Li H., Zhang Y., Wang Z., Ding J., Zeng Z, & Xia H. (2022). Hippocampal Mitochondrial Abnormalities Induced the Dendritic Complexity Reduction and Cognitive Decline in a Rat Model of Spinal Cord Injury. Oxidative Medicine and Cellular Longevity, 2022, 9253916.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!