Flow cytometric analysis was conducted to identify the phenotype of hDPSCs. The MSC phenotyping cocktail is comprised of both positive (CD29-FITC, CD44-PE, and CD90-PE-CY5, BD Bioscience, USA) and negative (CD34-PE and CD45-PE-CY5, BD Bioscience, USA) fluorochrome-conjugated monoclonal antibodies. IgG1-FITC, IgG1-PE-CY5 and IgG1-PE (BD Bioscience, USA) were used as isotype controls. hDPSCs were suspended to 4 × 105 cells/mL, incubated with different antibodies for 30 min at 4 °C, resuspended in FACS buffer, and analyzed using a MOFloTM high-performance cell sorter (Beckman Coulter, USA).
Cd90 pe cy5
Manufactured by BD
Sourced in United States
CD90-PE-CY5 is a fluorescently labeled antibody that targets the CD90 cell surface antigen. It is designed for use in flow cytometry applications to identify and characterize CD90-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 pe cy5, by BD (3 mentions)
Igg1 fitc, by BD (2 mentions)
Igg1 pe cy5, by BD (2 mentions)
Cd29 fitc, by BD (2 mentions)
Cd73 fitc, by BD (2 mentions)
Cd105 pe, by BD (2 mentions)
Cd34 pe, by BD (1 mentions)
Igg1 pe, by BD (1 mentions)
Cd44 pe, by BD (1 mentions)
Moflo xdp high performance cell sorter, by Beckman Coulter (1 mentions)
Cd14 fitc, by BD (1 mentions)
Cd34 pe, by Thermo Fisher Scientific (1 mentions)
Flow cytometry, by BD (1 mentions)
3 protocols using cd90 pe cy5
1
Phenotypic characterization of hDPSCs
2
Characterization of HDPC Surface Markers
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Surface markers of HDPCs were identified by flow cytometry. After collecting ~1×106 cells from the third passage, HDPCs were washed twice with PBS and then suspended in PBS, before being blocked with 5% BSA for 30 min at room temperature to remove nonspecific binding. The antibodies, including CD13-PE (1:40 dilution; cat. no. 555394; BD Biosciences), CD14-FITC (1:40 dilution; cat. no. 555397; BD Biosciences), CD29-FITC (1:40 dilution; cat. no. 11-0291-82; Thermo Fisher Scientific, Inc.), CD34-PE (1:40 dilution; cat. no. 560941; BD Biosciences), CD45-PE-Cy5 (1:40 dilution; cat. no. 560974; BD Biosciences), CD73-FITC (1:10 dilution; cat. no. 561254; BD Biosciences), CD90-PE-Cy5 (1:40 dilution; cat. no. 555597; BD Biosciences) and CD105-PE (1:40 dilution; cat. no. 560839; BD Biosciences), were added and cells were incubated for 90 min at 37°C. IgG1-PE (1:40 dilution; cat. no. 556650; BD Biosciences), IgG1-FITC (1:40 dilution; cat. no. 556649; BD Biosciences) and IgG1-PE-Cy5 (1:40 dilution; cat. no. 550618; BD Biosciences) were also used as isotype controls with incubation for 90 min at 37°C. After washing with PBS, suspended HDPCs were transferred to FACS tubes and analyzed using a MOFlo™ XDP high-performance cell sorter (Beckman Coulter, Inc.) and SUMMIT version 5.0 software (Beckman Coulter, Inc.) according to the manufacturer's instructions.
3
Characterization of hDPSCs by Flow Cytometry
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For identification analysis, hDPSCs were identified by the positive expression of MSC surface markers (CD73, CD90 and CD105) and the negative expression of hematopoietic antigens (CD34 and CD45). Human DPSCs at third passage were resuspended as single cell suspensions and incubated with fluorochrome-conjugated anti-human CD34-FITC, CD45-PE-CY5, CD73-FITC, CD90- PE-CY5, and CD105-PE (BD Biosciences, Franklin Lakes, NJ, USA) antibodies at 4 °C for 30 min. The samples were detected and analyzed by flow cytometry (BD Biosciences).
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