Ast and alt assay kits

Paederia scandens (Lour) Merr. (place of origin: Hubei, batch no. 181201) was purchased from Hainan Linshishengtai Pharmaceutical Co. Ltd. Acetaminophen tablets (batch no. 190402) were acquired from Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd. Silybin capsules (batch no. 850703059) were purchased from Tianjin Tasly Sants Pharmaceutical Co., Ltd. AST and ALT assay kits were obtained from Nanjing Jiancheng Bioengineering Institute. Rat ELISA kits of iNOS (LOT202005), IL-10 (LOT202006), IL-6 (LOT202006), TNF-α (LOT202006), and NF-κB (LOT202006) were obtained from Shanghai Enzyme-Linked Biotechnology Co., Ltd. Male Sprague Dawley rats were purchased from Changsha Tianqin Biological Technology Co., Ltd. Our study was performed according to the international, national, and institutional guidelines for animal experiments. The animal protocol was approved by the Ethics Review Committee for Animal Experimentation of Hainan Medical University. The ethical inspection number is HYLL-2021-387.

The content of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and total bile acid (TBA) in the serum was detected with commercially available kits (Jiancheng, Nanjing, Jiangsu, China) according to the manufacturer's protocols.
The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determinated using AST and ALT assay kits (Jiancheng, Nanjing, Jiangsu, China). The standard curve of enzymatic activity was drawn according to the manufacturer's instruction. After dilution, the serum sample (5 μL) was incubated with a substrate buffer (20 μL) at 37°C for 30 min, reacted with 2,4-dinitrophenylhydrazine buffer (20 μL) at 37°C for 20 min, and finally mixed with 0.4 mol/L NaOH (200 μL) at room temperature for 15 min. The optical density (OD) of the solution was measured at 510 nm. The AST and ALT activities were calculated based on the OD510.