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Lc ms vials

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LC-MS vials are specialized vessels used to hold and transport samples for analysis in liquid chromatography-mass spectrometry (LC-MS) instruments. These vials are designed to provide a secure and controlled environment for the samples during the analytical process.

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4 protocols using lc ms vials

1

Quantitative Urinary Acylcarnitine Profiling

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The acylcarnitine internal standard mix was made up of the following four deuterated acylcarnitines, each at a concentration of 200 μM: d3-acetylcarnitine, d3-butyrylcarnitine, d3-octanoylcarnitine, and d3-decanoylcarnitine. Ten μL of the acylcarnitine internal standard mix was spiked into 190 μL of human urine. Following the previous protocol, urine was dried down under nitrogen (N2) at 60°C and resuspended in 200 μL of methanol. Samples were vortexed and centrifuged at 14,000 rpm for 5 min. at room temperature. Urinary acylcarnitines were selectively isolated by solid-phase extraction (SPE) using SEP-PAK VAC 6 cc silica gel cartridges (Waters, Milford, MA, USA) following a literature procedure. This cartridge type is useful for acylcarnitines because it adsorbs analytes of even weak hydrophobicity from aqueous solution. Acylcarnitines were eluted off the SPE cartridges with 1.5 mL methanol:water:acetic acid (50:45:5, v/v/v). Extracts were then dried down under N2 and resuspended in 1 mL of methanol:water:formic acid (70:30:0.1, v/v/v). Samples were vortexed and centrifuged at 14,000 rpm for 5 min. at room temperature and ultimately transferred to LC-MS vials (Agilent, Palo Alto, CA, USA). Flow injection analysis (FIA) was utilized for sample introduction into the ESI-DMS-MS/MS platform.
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2

Radiation Effects on Contrast Agents

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The diluted contrast agent samples were placed in separate vials (2-mL clear glass liquid chromatography-mass spectrometry [LC-MS] vials [#5183–2067], Agilent Technologies, Santa Clara, CA, USA). Each vial was placed at the isocenter of a 1.5-T Unity MR-Linac (Elekta, Crawly, UK) and irradiated with 7 MV photons to doses of approximately 2 Gy, 8 Gy, 15 Gy, or 30 Gy; two non-irradiated vials were used as baselines for comparison (controls).
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3

Acetylcarnitine Quantification in NHP Urine

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Firstly, 5.26 μL of 120 μM deuterated acetylcarnitine internal standard solubilized in water (100%) was spiked into 100 μL of NHP urine (final concentration of IS = 6 μM), dried down under nitrogen (N2) at 60°C, resuspended in 105 μL of methanol, vortexed and centrifuged at 14,000 rpm for 5 minutes at room temperature. Acetylcarnitine was then extracted by solid-phase extraction using SEP-PAK VAC 6cc silica gel cartridges (Waters, Milford, MA, USA). Acetylcarnitine was eluted with 1.5 mL methanol:water:acetic acid (50:45:5, v/v/v)38 . The extract was then dried down under N2, resuspended in 526.3 μL of methanol:water:formic acid (70:30:0.1, v/v/v), vortexed, centrifuged at 14,000 rpm for 5 minutes and transferred to LC-MS vials (Agilent, Palo Alto, CA, USA). Flow injection analysis (FIA) enabled sample introduction into the mass spectrometer for DMS-MS/MS analysis (Figure 1).
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4

Steroid Derivatization for LC-MS

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Derivatization was based on previous studies (Keski-Rahkonen et al., 2015 (link); Handelsman et al., 2020 ). Here, the protocol was slightly modified to reduce reagent evaporation. Dried extracts were immersed in an ice bath, then samples were reconstituted with 30 μl of sodium bicarbonate buffer (50 mm, pH 10.5), briefly vortexed, and 20 μl of 1 mg/ml DMIS in acetone was added. Samples were then vortexed and centrifuged at 3200 × g for 1 min before being transferred to glass LC-MS vial inserts placed in LC-MS vials (Agilent). Vials were capped to prevent evaporation during incubation for 15 min at 60°C. This was followed by a cooling period of 15 min at 4°C. Samples were centrifuged at 3200 × g for 1 min, and then stored at −20°C for no more than 24 h before steroid analysis.
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