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3 protocols using 3 4 methylenedioxymethamphetamine

1

Detection and Quantification of Illicit Drugs

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Chloroform (CHCl3) and ethyl acetate (EA) were purchased from Macron Fine Chemicals (Center Valley, PA, U.S.A.). Formic acid and methanol were obtained from Sigma–Aldrich(St. Louis, MO, U.S.A.) and Merck (Darmstadt, Germany), respectively. Ethanol and acetic acid were purchased from J.T. Baker (Phillipsburg, NJ, U.S.A.). Distilled deionized water was produced by a PURELAB purifier (ELGA, Marlow, U.K.). Standards for illegal drugs including 5-MeO-AMT, amphetamine (AM), methamphetamine (MA), mephedrone, para-methoxymethamphetamine (PMMA), 3,4-methylenedioxymethamphetamine (MDMA), methylone, 2-(4-chloro-2,5-dimethoxyphenyl)ethan-1-amine (2C-C), ketamine, nitrazepam, flunitrazepam (FM2), and lysergic acid diethylamide (LSD) were purchased from Cerilliant Corporation (Round Rock, TX, U.S.A.). Stock solutions of illicit drugs were prepared in MeOH. Drinks and powders for whole fat milk (Uni-President Enterprises Corporation), grape juice (Uni-President Enterprises Corporation), fruit and vegetable juice (Chou-Chin Industrial Co.), sugar-free green tea bags (Vitalon Foods Company), 3-in-1 instant coffee packs (Nestlé Company), matcha tea packs (Starbucks), and gelatin powder (Tehmag Foods Corporation) were obtained from local supermarkets. Postage stamps were purchased from Chunghwa Post Co., Taiwan.
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2

Quantitative Analysis of Illicit Drugs and Novel Psychoactive Substances

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LC–MS-grade methanol (purity, ≥99.9%) was purchased from Merk (Darmstadt, Germany). HPLC-grade acetonitrile (purity, ≥99.9%) was purchased from J.T. Baker (Phillipsburg, NJ, USA). Ten standards of illicit drugs, methamphetamine (1 mg/mL), amphetamine (1 mg/mL), ketamine (1 mg/mL), norketamine (1 mg/mL), morphine (0.1 mg/mL), codeine (1 mg/mL), Tetrahydrocannabinol (THC, 1 mg/mL), 11-nor-9-carboxy-THC (THC-COOH, 0.1 mg/mL), 3,4-methylenedioxymethamphetamine (MDMA, 1 mg/mL), mephedrone (1 mg/mL), flunitrazepam (1 mg/mL), 7-aminoflunitrazepam (1 mg/mL), nimetazepam (1 mg/mL), 7-aminonimetazepam (1 mg/mL), and zopiclone (1 mg/mL) were purchased from Cerilliant (Round Rock, TX, USA). Three standards of NPSs, 4′-chloro-alpha-pyrrolidinopropiophenone (4-Cl-α-PPP, 1 mg, purity, ≥98%), 4′-Fluoro-α-pyrrolidinopentiophenone (4-F-α-PVP, 1 mg, purity, ≥98%), and 1-pentyl-3-(2-bromophenylacetyl)indole (JWH-249, 1 mg, purity, ≥98%) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Sodium azide (500 mg, purity, ≥99%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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3

Comprehensive Synthetic Cathinone Analysis

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The stock standard solutions of amphetamine (A), methamphetamine (MA), phenylpropanolamine (PHPR), ephedrine, pseudoephedrine (PSE), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), phentermine (PHRM), methylphenidate (MPHD), bupropion, hydroxybupropion, methcathinone (ephedrone), methedrone (PMMC), mephedrone (4-MMC), methylone (MDMC), ethylone (MDEC), 3,4-methylenedioxypyrovalerone (MDPV), and alpha-pyrrolidinopentiophenone (alpha-PVP) at a concentration of 1.0 mg mL -1 for each analyte in methanol were purchased from Cerilliant (Round Rock, Texas, USA). Stock standard solutions of the internal standards (ISTDs), namely amphetamine-d11, methamphetamine-d11, ephedrine-d3, pseudoephedrine-d3, norpseudoephedrine-d3, MDA-d5, MDMA-d5, MDEA-d5, phentermine-d5, methylphenidate-d9, bupropion-d9, hydroxybupropion-d6, mephedrone-d3, and methylone-d3, were also purchased from Cerilliant (Round Rock, TX, USA) at a concentration of 100 µg mL -1 in methanol.
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