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Sinergymx plate reader

Manufactured by Agilent Technologies
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The SinergyMx plate reader is a multi-mode microplate reader from Agilent Technologies. It is designed to perform absorbance, fluorescence, and luminescence measurements in microplates for a variety of applications in life science research and drug discovery.

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Lab products found in correlation

Facscanto, by BD (1 mentions) Facscelesta flow cytometer, by BD (1 mentions) Jc 10 dye, by Abcam (1 mentions)

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SinergyMx (8 citations)

2 protocols using sinergymx plate reader

1

Caspase-1 Inhibitor Drug Screening

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Potential inhibitors of caspase-1 were analyzed using Caspase-1 Inhibitor Drug Screening Kit (Fluorimetric) from BioVision. Shikonin and positive inhibition control (Z-VAD-FMK) were prepared in PBS and applied to black 96-well fluorescence plate (Corning). Active caspase-1 was added, followed by caspase-1 substrate YVAD-AFC. After 45 min incubation at 37°C, fluorescence of samples was measured using SinergyMx plate reader (Biotek).
Zorman J., Sušjan P, & Hafner-Bratkovič I. (2016). Shikonin Suppresses NLRP3 and AIM2 Inflammasomes by Direct Inhibition of Caspase-1. PLoS ONE, 11(7), e0159826.
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2

Mitochondrial Membrane Potential Assay

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Mitochondrial membrane potential was measured using the JC-10 dye (Abcam) in BMDMs with a Sinergy Mx plate reader (BioTek) or by flow cytometry using the BD FACS Celesta flow cytometer (BD Biosciences), or in human monocytes (CD14+ labeled with anti-CD14 APC-H7, clone MφP9, catalog 560180, BD Biosciences) using FACS Canto (BD Biosciences), following the indications of manufacturer.
Martínez-García J.J., Martínez-Banaclocha H., Angosto-Bazarra D., de Torre-Minguela C., Baroja-Mazo A., Alarcón-Vila C., Martínez-Alarcón L., Amores-Iniesta J., Martín-Sánchez F., Ercole G.A., Martínez C.M., González-Lisorge A., Fernández-Pacheco J., Martínez-Gil P., Adriouch S., Koch-Nolte F., Luján J., Acosta-Villegas F., Parrilla P., García-Palenciano C, & Pelegrin P. (2019). P2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis. Nature Communications, 10, 2711.
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