Scil vet abc plus

Thirty-three male Wistar rats weighing 182–212 g were randomly assigned to 3 groups, anesthetized by an intraperitoneal injection of pentobarbital (45 mg kg⁻¹), and placed in a supine position on a heated operating table. Rats were intravenously injected into the right femoral vein with HBC (4.5 mg kg⁻¹) 5 min after injection with HS (6 mg kg⁻¹). Twenty minutes after HS administration, the blood samples were collected from the heart and drawn into 3.13% trisodium citrate. Neutralization of HS by HBC was monitored by measuring aPTT, PT, anti-fXa and anti-fIIa activity, and platelet aggregation. Coagulation parameters were determined as described above whereas platelet aggregation was measured after incubation of sodium citrate-anticoagulated blood with 0.9% NaCl solution (volume ratio 1 : 1) for 20 min at 25 °C and then for 15 min at 37 °C. The changes in impedance registered as aggregation curve were recorded for 6 min after collagen addition (7.5 μg mL⁻¹) using a Chrono-log aggregometer (Chrono-log Corp.). Four parameters were automatically calculated: maximal extension (MaxA), lag phase (Lag), the slope of platelet aggregation (Slp), and area under the curve (AUC). The white blood cell count (WBC) was automatically performed using an animal blood counter (scil Vet abc Plus, Horiba, France).