Expression of transforming growth factor beta one (TGF-β1) in Transwell “conditioned” media was determined using ELISA assay kits (Invitrogen). Briefly, media in the Transwell® were collected at 24 and 48 h following SHI treatment. The ELISA assay was then performed as per the manufacturer’s instructions. Expression of TGF-β1 was measured at 450 nm using a Microplate Reader (Optima).
Elisa assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ELISA assay kit is a laboratory equipment designed for enzyme-linked immunosorbent assays (ELISA). The core function of this kit is to detect and quantify specific analytes, such as proteins, hormones, or antibodies, in a sample. The kit typically includes the necessary reagents, plates, and instructions to perform the ELISA procedure.
Automatically generated - may contain errors
Lab products found in correlation
Infinite m200, by Tecan (4 mentions)
Duoset elisa, by R&D Systems (2 mentions)
Protease and phosphatase inhibitor, by Roche (2 mentions)
Microplate reader, by Bio-Rad (2 mentions)
Synergy h4 hybrid multi mode microplate reader, by Agilent Technologies (1 mentions)
Biotek elx808, by Agilent Technologies (1 mentions)
Confocal laser scanning microscope, by Leica (1 mentions)
Novex elisa kit, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
Bms213 2, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg h l hrp, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg h l hrp, by Thermo Fisher Scientific (1 mentions)
Anti occludin, by Affinity Biosciences (1 mentions)
Tabersonine, by Merck Group (1 mentions)
Spectramax i3, by Molecular Devices (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Zymo Research (1 mentions)
41 protocols using elisa assay kit
1
Quantifying TGF-β1 in Transwell Media
2
Cerebral Organoid Protein Analysis
3
Measuring Inflammatory Cytokines in Mouse Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood of mice was collected through eyeball extraction after co-treatment with LPS and IBU, and serum was separated through centrifugation at 3500 rpm at 4°C for 15 min.
The levels of TNF-α, IL-1β, IL-6, and MCP-1 in serum were measured using ELISA assay kits (Invitrogen, Carlsbad, CA) in accordance with the manufacturer’s instructions. The color intensity was assayed at 450 nm with reference wave length using a microplate reader (Bio-Rad, Hercules, CA).
The levels of TNF-α, IL-1β, IL-6, and MCP-1 in serum were measured using ELISA assay kits (Invitrogen, Carlsbad, CA) in accordance with the manufacturer’s instructions. The color intensity was assayed at 450 nm with reference wave length using a microplate reader (Bio-Rad, Hercules, CA).
4
Quantifying IL-8 Levels in Intestinal Cell Co-culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of IL-8 produced by mono-cultured Caco-2 or EA.hy926 cells in the Caco-2 BBe1/EA.hy926 co-culture model was determined using ELISA assay kits (Invitrogen, Carlsbad, California, United States). The procedures of the measurement were carried out according to the manufacturer’s instructions. In general, 100 μL of the supernatant of culture medium was used for IL-8 assays. After the reactions for 30 min, the microplates were read at 450 nm using a Synergy™ H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, Vermont, United States). Final concentrations of IL-8 were calculated from a calibration curve, and the IL-8 levels were presented as pg/mL.
5
Serum AKP and Plasma LPS Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AKP activity in serum was determined using commercial reagent kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). LPS level in plasma was determined using the commercially available Tachypleus amebocyte lysate kit (Chinese Horseshoe Crab Reagent Manufactory Co., Ltd, Xiamen, China) following the quantitative Chromogenic Limulus Amebocyte Lysate assay method.
Protein expression levels of IL-8, IL-6, and TNF-α proteins in the colonic mucosa were determined using ELISA assay kits (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. A microplate reader (BioTek-ELx808, BioTek Instruments, Inc., Texas) was used to read the protein bands.
Protein expression levels of IL-8, IL-6, and TNF-α proteins in the colonic mucosa were determined using ELISA assay kits (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. A microplate reader (BioTek-ELx808, BioTek Instruments, Inc., Texas) was used to read the protein bands.
6
Cerebral Organoid Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Individual cerebral organoids were homogenized in an icecold RIPA buffer containing protease and phosphatase inhibitors (Roche, Switzerland), following centrifugation at 14,000 rpm and 4 °C for 10 min. The protein concentration of the supernatant was determined by BCA (Pierce, WI, USA). Total and phospho-tau levels, as well as IL-1β and TNF-α levels, were assessed, in equal protein amounts, using ELISA assay kits (total-tau #KHB0041 and phoshotau #KHO0631, both Invitrogen (MA, USA); IL-1β #DY201 and TNF-α #DY210, both DuoSet ELISA, R&D Systems, MN, USA), according to the manufacturer’s instructions.
7
Inflammatory Cytokine Measurement in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After cotreatment of LPS and narciclasine, blood samples of each group were collected through eyeball extraction, and serum samples were obtained through centrifugation at 4000 rpm for 10 min at 4°C. The expressions of tumor necrosis factor α (TNF-α) interleukin 6 (IL-6), interleukin 1β (IL-1β), and monocyte chemotactic protein-1 (MCP-1) in serum were determined using ELISA assay kits (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The color intensity was assayed at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA).
8
Pulmonary Inflammatory Marker Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin-fixed, paraffin-embedded tissue sections of lung tissues were obtained from treatment and control groups as described above in animal grouping and treatment. The expression of rBD-2 was detected following the immunohistochemistry protocol of Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). The sections were incubated with primary rabbit polyclonal antibody rBD-2 (YY3068R; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 1:200 dilution overnight at 4°C and secondary antibody [SP9000D; DAB Detection kit (Streptavidin-biotin); ZSGB-BIO, Beijing, China] for another 2 h at room temperature. Finally, visualization of the sections was conducted and images were taken with a Leica confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany).
Determination of IL-6 and tumor necrosis factor (TNF)-α expression in lung tissues and peripheral blood. At 24 h following ATP and cephalosporin intervention, rats from control and treatment groups were sacrificed and lung tissues were isolated, centrifuged at 2,045 × g for 20 min at room temperature followed by collection of supernatants. The peripheral blood samples were also collected. IL-6 and TNF-α expression was evaluated using ELISA assay kits in accordance with the instructions (Invitrogen; Thermo Fisher Scientific, Inc.).
Determination of IL-6 and tumor necrosis factor (TNF)-α expression in lung tissues and peripheral blood. At 24 h following ATP and cephalosporin intervention, rats from control and treatment groups were sacrificed and lung tissues were isolated, centrifuged at 2,045 × g for 20 min at room temperature followed by collection of supernatants. The peripheral blood samples were also collected. IL-6 and TNF-α expression was evaluated using ELISA assay kits in accordance with the instructions (Invitrogen; Thermo Fisher Scientific, Inc.).
9
Quantification of Inflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of IL-6, IL-12, IL-17, TNF-alpha and IFN-gamma were quantified by ELISA assay kits (Thermo Fisher Scientific, MA, USA) in serum samples according to the manufacturer’s instructions. Detection limits were: 7.8–500 pg/mL for IL-6, 1.56–100 pg/mL for IL-12, 0.23–15 pg/mL for IL-17, 15.6–1000 pg/mL for TNF-alpha and 1.6–100 pg/mL for IFN-gamma. IL-10 cytokine was measured by Novex® ELISA Kits (Life Technology, ES, Carlsbad, CA, USA), performed according to the manufacturer’s instructions. The assay range was 7.8–500 pg/mL for IL-10.
10
Fasting Blood Biomarker Levels Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood samples at baseline were acquired from all patients after at least 12 hours of fasting. Additionally, more peripheral blood samples were acquired at 30 minutes, 60 minutes, 90 minutes, 120 minutes, 150 minutes, and 180 minutes after the patients took a standard meal as described above. All peripheral blood samples were acquired using ice‐cold blood collection tubes containing a final concentration of 1.0 mg/mL of EDTA and 500 KIU/mL of aprotinin (Sigma Aldrich). The contents of neurotensin, CRF, motilin and somatostatin in each peripheral blood sample were determined by ELISA assay kits (Thermo Fisher Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!