V8031

The following primary antibodies were used for immunostaining or Western blot analysis: anti-mouse myelin basic protein (MBP; 1:300, #SMI-94R [Covance]), anti-rabbit Krox-20 (1:500, #PRB-236P [Covance]), anti-mouse BrdU (1:1000, #B2531 [Sigma]), anti-rabbit phospho-Akt (1:1000, #4051 [Cell Signaling Technology]), anti-rabbit phospho-Erk1/2 (1:5000, #V8031 [Promega]), anti-rabbit total-Akt (1:1000, #9272 [Cell Signaling Technology]), anti-rabbit total Erk1/2 (1:5000, #V1141 [Promega]), anti-rabbit phospho-ErbB3 (1:500, #4791 [Cell signaling]), anti-rabbit laminin (1:1000, #ab11575 [Abcam]), anti-mouse β-actin (1:5000, #A5441 [Sigma]), and anti-rabbit TIMP-3 (1:1000, #AB6000 [Millipore]).

Used primary antibodies were used: mouse monoclonal anti-GFP (1814460; Roche Diagnostics, Basel, Switzerland); polyclonal serum (rabbit) recognizing the mitogen-activated protein kinase (MAPK) phosphorylated TEY motif (V8031; Promega, Madison, WI)^{13 (link)}; sheep polyclonal anti-phosphoTyr¹⁷¹ CDKL5^{22 (link)} and sheep polyclonal anti-phosphoSer⁹⁰⁰ MAP1S^{22 (link)} (both obtained from MRC-PPU Reagents, University of Dundee, Dundee, United Kingdom); monoclonal anti-CDKL5 (mouse; sc-376314; Santa Cruz Biotechnologies, Dallas, TX); and polyclonal anti-RFP (rabbit; PM005; MBL International, Woburn, MA).