We examined the morphological modifications on treated cells, compared to untreated control cells. Cells were seeded in 6 well plates using the RPMI medium. After incubation in a humidified 37 °C, 5% CO2 incubator (Series II Water Jacket supplied by Thermo Scientific) overnight, cells were then treated with respective clam extracts (600 μg/ml) and incubated for 72 h. Cell morphology was observed under an inverted microscope (Zeiss Primotech microscope), at 40 x magnification.
Primotech microscope
Manufactured by Zeiss
The Primotech is a high-quality microscope designed for laboratory use. It features a stable and durable construction, providing reliable performance for a wide range of applications. The Primotech offers essential microscopic observation capabilities, allowing users to examine samples with clarity and precision.
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7 protocols using primotech microscope
1
Morphological Changes in Treated Cells
2
Cellulose Acetate Electrospun Scaffolds
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Solutions of CA (15, 16, 17, and 18% wt) were prepared for electrospinning. A 10 ml syringe (Henke-Sass Wolf) with a 21G 0.8 mm × 50 mm needle (BD Microlance) was loaded with the solutions and connected to a microfluidics syringe pump (NE-1002X, ALA Scientific Instruments). The pump was programmed to dispense a flow rate of 1 ml/h. The electric field was generated by a power supply (Linari Engineering) connected to both the syringe needle and the collector through an applied voltage of 12 kV. A stainless-steel woven wire mesh with 1 mm2 holes was positioned at 10 cm distance from the needle tip and was used as support for the electrospun CA fibers. CA solutions with 16, 17, and 18% wt cellulose resulted highly viscous, and electrospinning resulted not possible in these conditions. For this reason, CA electrospun scaffolds were prepared with the 15% wt cellulose solution; three different spinning times (5, 15, and 25 min) were tested and characterized. Optical imaging was carried out with a Zeiss Primotech microscope and SEM imaging was performed as described above. Porosity and fiber size were measured with ImageJ. The optical transmittance of electrospun CA scaffolds (25 min of electrospinning), CA membranes obtained via VIPS, and commercially available PET membranes was investigated in the range of 300–800 nm by a spectrophotometer Cary 5000 (Agilent).
3
Eggshell Microscopic Examination Protocol
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A piece of eggshell was removed from YLSNHM 01373 with an Engraving Pen AT-310. The shell was embedded in Araldite 2020, cut with a STX-202A diamond wire cutting machine, and then polished with P400 to P4000 abrasive paper to approximately 30 μm thick for microscopic observation under normal and cross-polarized light with a Zeiss Primotech microscope.
4
Petrographic Analysis of Rock Samples
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Thin sections were examined and described under normal and polarized light with a lambda compensator using a Zeiss PrimoTech microscope. Images were captured using an integrated microscope camera at 200x magnification. Photographs and EDS graphs were compiled using Adobe Photoshop CS6 and Adobe Illustrator CS6. Coloration was determined using the Munsell soil color chart.123
5
Comprehensive Urine Analysis Protocol
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The first morning urine was collected by the owner into a sterile container during spontaneous urination. The test was carried out immediately after receiving urine samples. The following parameters were evaluated during the physicochemical examination of urine: Colour, transparency, specific gravity, pH, protein, albumin, creatinine, glucose, blood, acetone and urobilinogen. The urine sediment was obtained through centrifugation for 10 min at 2500× g. Low speed spinning prevented podocyte damaged. Cell elements in the urine sediment were detect by a ZEISS Primotech microscope. The number of epithelial cells, blood cells, casts, and bacteria were assessed. A total of 0.7 mL of the urine sediment was stored at −80 °C until it was transferred to the Faculty of Chemistry, University of Wroclaw, Poland, in order to perform LC-MS-MRM. UPC was determined in the supernatant, and the UAC was calculated by dividing the protein albumin concentration (mg/dL) by the urine creatinine concentration (mg/dL). The result is a unitless ratio (dimensionless).
6
Microscopic Analysis of Tooth Formation
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Images of the thin-sections were observed and traced using a Zeiss Primotech microscope at 10x or 20x magnification. In thin–sections, incremental lines of von Ebner are ubiquitous light/dark couplets that record the daily apposition of dentine in teeth [36 ]. The number of incremental lines of von Ebner indicates tooth formation time, whereas their thickness indicates the rate of dentine formation per day [37 , 38 (link)]. Measurements from thin-sections were taken using ImageJ v. 1.53 [39 (link)] to get the daily dentine apposition rate (DDAR) and the counts of incremental lines. We followed the methods and arguments laid out in D’Emic, O’Connor [40 (link)] in identifying von Ebner’s incremental width.
7
Comprehensive Urine Analysis Protocol
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The urine examination was carried out immediately after receiving the urine samples (via cystocentesis) to exclude kidney and urinary tract disease. A physicochemical examination of urine included an analysis of its color, transparency, specific gravity, pH, protein, albumin, creatinine, glucose, blood, acetone, and urobilinogen levels. The urine sediment was obtained through centrifugation for 10 min at 2500× g. The urine sediment was detected using a ZEISS Primotech microscope (Oberkochen, Gremany), measuring the numbers of epithelial cells, blood cells, casts, and bacteria.
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