A1rsi inverted microscope

Manufactured by Nikon

The A1Rsi inverted microscope is a high-performance imaging system designed for advanced biological and materials research. It features a robust and stable optical configuration, along with precision control and advanced imaging capabilities. The A1Rsi is capable of delivering high-quality, high-resolution images to support a wide range of scientific investigations.

Automatically generated - may contain errors

Lab products found in correlation

Ti e inverted microscope, by Nikon (1 mentions) Neurolucida software, by MicroBrightField (1 mentions)

3 protocols using a1rsi inverted microscope

Quantifying Hippocampal Neuronal Populations

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Confocal image stacks through the z-depth of the hippocampal dentate hilus were collected using a Nikon A1Rsi inverted microscope with a 40× water objective (NA 1.15, resolution 0.62 μm/pixel). Image stacks were used to assess the number of granule cells (Prox1) and mossy cells (GluR2/3) in the dentate hilus (XY dimensions = 1818 μm × 512 μm, z-depth = 20 μm with a 0.4μm step). For each mouse a total of 3–4 hilar regions were imaged at bregma level −2.0 to −2.3. Hilar ectopic granule cells were defined as being Prox1 immunopositive cells located within the hilus and at least 20 μm from the granule cell layer-hilar border. Mossy cells were defined as GluR2/3 immunopositive cells located within the hilus with a soma diameter >20 μm (Jiao and Nadler, 2007 (link)). Cell counts were performed using a modified optical dissector method (Howell et al., 2002 (link); Hofacer et al., 2013 (link)). Results are reported as hilar Prox1 or GluR2/3 cell densities (Prox1 or GluR2/3 cells/ x1000 mm³ of hilus).

Wulsin A.C., Kraus K.L., Gaitonde K.D., Suru V., Ragab S., Packard B.A., Herman J.P, & Danzer S.C. (2021). The glucocorticoid receptor specific modulator CORT108297 reduces brain pathology following status epilepticus. Experimental neurology, 341, 113703.

+ Open protocol

+ Expand

Cellular Fractionation and Immunoassays

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblot assays, equal aliquots of nuclear, cytoplasmic, or whole-cell lysates were resolved by SDS-PAGE and transferred to PVDF membranes using standard methods. Nuclear and cytoplasmic fractions were generated via a differential lysis using Buffer A (10 mM HEPES; pH 7.9, 10 mM KCL, 0.1 mM EDTA, and protease and phosphatase inhibitors) to obtain a cytoplasmic fraction. Intact nuclei were pelleted from these lysates and washed twice in fresh Buffer A before being lysed in Buffer B (20 mM HEPES; pH 7.9, 0.4 M NaCl, 1 mM EDTA, 10% glycerol, and protease and phosphatase inhibitors). Immunofluorescent assays were conducted using primary antibodies in combination with fluorescently labeled secondary antibodies. Confocal images were captured using a Nikon A1Rsi inverted microscope. Super-resolution images are structure illuminations obtained on a Nikon Ti-E inverted microscope with N-SIM capability. Antibody concentrations and suppliers are listed in Supplementary Table 3.

Ali R., Brown W., Purdy S.C., Davisson V.J, & Wendt M.K. (2018). Biased signaling downstream of epidermal growth factor receptor regulates proliferative versus apoptotic response to ligand. Cell Death & Disease, 9(10), 976.

+ Open protocol

+ Expand

Identifying PTEN Knockout Cells in Hippocampus

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Biocytin-filled cells were imaged using a Nikon A1Rsi inverted microscope (software RRID:SCR_014329) equipped with a 40× Plan Apo water immersion objective (NA=1.15; field size 317 × 317 μm; 0.5 μm z-step). Confocal z-series image stacks were imported to Neurolucida software (Microbrightfield Inc., RRID: SCR_001775) for whole cell tracing. Overlapping image stacks were three-dimensionally montaged into a single image for reconstruction. Reconstructions encoded soma area, dendritic length and branch points. Image stacks were also used to encode the location of the hilar-granule cell body layer border, the granule cell body layer-molecular layer border, and the location of the hippocampal fissure, as described in prior work (Santos et al. 2011 (link)). Since KO animals are mosaics, the blind patch approach yielded both PTEN KO and PTEN-expressing cells. Cells were categorized as either PTEN KO or PTEN-expressing only after reconstruction using soma area as a criterion. Cells with soma areas that exceeded two standard deviations of the control cell mean for biocytin-filled cells (107.8±18.3) were defined as KOs; and were not included in the present study. Cells below this size threshold were defined as PTEN-expressing for the current work. This criteria has been previously validated to distinguish >95% of KO cells from control cells (Santos et al. 2017 (link)).

LaSarge C.L., Pun R.Y., Gu Z., Santos V.R, & Danzer S.C. (2019). Impact of mTOR hyperactive neurons on the morphology and physiology of adjacent neurons: Do PTEN KO cells make bad neighbors?. Experimental neurology, 321, 113029.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!