Milli qtm system

All laboratory reagents used in the experiments were of analytical grade. The deionised water (d.i.H₂O) utilised for the experiments was procured from a MilliQ^TM system (Millipore Australia Pty Ltd., Sydney, NSW, Australia). Phen, 5-Mephen, 5,6-Me₂phen, NPX, ACE, N,N′-dicyclohexylcarbodiimide (DCC), dimethyl sulfoxide (DMSO), acetonitrile (CH₃CN) and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich, Sydney, NSW, Australia. Sep-Pak^® C₁₈-reverse phase columns were purchased from Waters Australia Pty Ltd., Sydney, NSW, Australia. Deuterated oxide 99.9% (D₂O) and deuterated DMSO (DMSO-d₆) were purchased from Cambridge Isotope Laboratories, Andover, MA, USA. Methanol (MeOH) was obtained from Honeywell Research Chemicals, NJ, USA. Diethyl ether (Et₂O) and acetone (C₃H₆O) were purchased from ChemSupply Australia, Gillman, SA, Australia. Additional laboratory reagents were acquired from commercial sources.

All chemicals and reagents were of analytical grade and used without further purification. The deionised water (d.i.H₂O) used for the experiments was obtained from a MilliQ^TM system (Millipore Australia Pty Ltd., Sydney, NSW, Australia). Potassium tetrachloroplatinate(II), SS-DACH, phen, 5,6-Me₂phen, 4-CPA, 4-FPA, 4-BPA, N,N’-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), dimethyl sulfoxide (DMSO), acetonitrile (CH₃CN), trifluoroacetic acid (TFA), AsA, MF-Millipore^TM membrane filters (25 mm, 0.45 µm), and Whatman^® (55 mm) cellulose papers were purchased from Sigma-Aldrich, Sydney, NSW, Australia. 4-IPA was purchased from Combi-Blocks Inc, San Diego, CA, USA. Sep-Pak^® C₁₈-reverse phase columns were procured from Waters Australia Pty Ltd., Sydney, NSW, Australia. Advantec^® (55 mm) cellulose filter papers were purchased from Sterlitech Corporation, Auburn, WA, USA. Deuterium oxide 99.9% (D₂O) and deuterated acetonitrile (CD₃CN) were purchased from Cambridge Isotope Laboratories, Andover, MA, USA. Methanol (MeOH) was obtained from Honeywell Research Chemicals, NJ, USA. Ethanol (EtOH), diethyl ether (Et₂O), acetone (C₃H₆O), and dichloromethane (DCM) were purchased from ChemSupply, Gillman, SA, Australia. All other chemicals and certain goods were obtained from commercial sources.

C-terminal amidated PuroA (FPVTWRWWKWWKG-NH₂) and FITC-labelled PuroA (FITC-FPVTWRWWKWWKG-NH₂) were synthesized with >95% purity by solid-phase methods using N-(9-fluorenyl) methoxycarbonyl (Fmoc) chemistry at Biomatik Crop (Ontario, CA). Peptides used without further purification. Peptide solutions were made in sterile phosphate-buffered saline (PBS; 140 mM NaCl, 2.5 mM KCl, 1.6 mM KH₂PO₄, 15 mM Na₂HPO₄, pH 7.4). A 20 mM solution (in DMSO) of propidium iodide and A 5 mM solution (in DMSO) of SYTO 85 Orange fluorescent nucleic acid stain were purchased from Molecular Probes (L7012 and S11366, respectively). All aqueous solutions were made in sterile, ultrapure (18 MΩ) water which was quartz-distilled and deionised in a Milli-Q^TM system (Millipore, Bedford, MA, USA).

All chemicals and reagents were of spectroscopic grade and used without further purification. The deionized water (d.i.H₂O) used for the experiments was obtained from a MilliQ^TM system (Millipore Australia Pty Ltd, Sydney, NSW, Australia). Potassium tetrachloroplatinate(II) (K₂PtCl₄), S,S-DACH, 1,10-phenanthroline, 5-methyl-1,10-phenanthroline, 5,6-dimethyl-1,10-phenanthroline, CLB, N,N′-dicyclohexylcarbodiimide (DCC), dimethyl sulfoxide (DMSO), acetonitrile (CH₃CN), trifluoroacetic acid (TFA), silver nitrate (AgNO₃⁻), ascorbic acid (AsA) and 30% hydrogen peroxide (H₂O₂) were purchased from Sigma-Aldrich, Sydney, NSW, Australia. Sep-Pak^® C₁₈-reverse phase columns were purchased from Waters Australia Pty Ltd, Sydney, NSW, Australia. Deuterated solvents such as deuterium oxide 99.9% (D₂O) and deuterated acetonitrile (CD₃CN) were purchased from Cambridge Isotope Laboratories, Andover, MA, USA. Methanol (MeOH) was obtained from Honeywell Research Chemicals, NJ, USA. Ethanol (EtOH), diethyl ether (Et₂O), ethyl acetate (EtOAc) and acetone were purchased from ChemSupply, Gillman, SA, Australia. All other chemicals, reagents, and lab consumables were purchased from commercial sources.