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96 dynamic array ifc

Manufactured by Standard BioTools
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The 96 Dynamic Array IFC is a microfluidic device designed for high-throughput gene expression analysis. It enables the simultaneous measurement of up to 96 target genes across 96 samples, facilitating efficient and scalable genomic research. The device is compatible with various real-time PCR platforms and is a core component of the Standard BioTools product portfolio.

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Lab products found in correlation

Biomark system, by Standard BioTools (2 mentions) Dna binding dye sample loading reagent, by Standard BioTools (2 mentions) Assay loading reagent, by Standard BioTools (2 mentions) Superscript 3 cells direct cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Ssofast evagreen supermix with low rox, by Bio-Rad (2 mentions) Dna suspension buffer, by Teknova (2 mentions)

Spelling variants of this product

96.96 Dynamic Arrays (36 citations) Dynamic Array IFC (4 citations) Dynamic Arrays (2 citations)

2 protocols using 96 dynamic array ifc

1

Single-Cell Gene Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Classical monocytes and naive T cells were sorted directly into 96-well plates containing Cells Direct 2x Reaction Mix, with 200 cells per well in replicates of 6. Lysed cells were subjected to reverse transcription and gene-specific pre-amplification using the SuperScript® III CellsDirect cDNA Synthesis Kit (Invitrogen). Primers were purchased from IDT and the specific targeting amplification (STA) mix was prepared according to the Fluidigm protocol (Gene Expression Using SsoFast EvaGreen SuperMix with Low ROX on the BioMark or BioMark HD System). Unincorporated primers were digested with Exonuclease I (New Englad Biolabs). Amplified cDNA was diluted and combined with 2X SsoFast EvaGreen Supermix with Low ROX (Bio-rad) and 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). Primers were combined with 2X Assay Loading Reagent (Fluidigm) and 1X DNA Suspension Buffer (Teknova). Sample and primer mixes were loaded onto 96.96 Dynamic Array IFC (Fluidigm) and qPCR was performed using the Bio-Mark System (Fluidigm).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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2

Single-Cell Gene Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Classical monocytes and naive T cells were sorted directly into 96-well plates containing Cells Direct 2x Reaction Mix, with 200 cells per well in replicates of 6. Lysed cells were subjected to reverse transcription and gene-specific pre-amplification using the SuperScript® III CellsDirect cDNA Synthesis Kit (Invitrogen). Primers were purchased from IDT and the specific targeting amplification (STA) mix was prepared according to the Fluidigm protocol (Gene Expression Using SsoFast EvaGreen SuperMix with Low ROX on the BioMark or BioMark HD System). Unincorporated primers were digested with Exonuclease I (New Englad Biolabs). Amplified cDNA was diluted and combined with 2X SsoFast EvaGreen Supermix with Low ROX (Bio-rad) and 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). Primers were combined with 2X Assay Loading Reagent (Fluidigm) and 1X DNA Suspension Buffer (Teknova). Sample and primer mixes were loaded onto 96.96 Dynamic Array IFC (Fluidigm) and qPCR was performed using the Bio-Mark System (Fluidigm).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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