Anti plk1 antibody

Manufactured by Proteintech

Sourced in China

Anti-PLK1 antibody is a laboratory-grade reagent designed for the detection and study of the Polo-like kinase 1 (PLK1) protein. PLK1 is a serine/threonine-protein kinase that plays a crucial role in the regulation of cell division and mitosis. This antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and localization of PLK1 in biological samples.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Mg132, by Merck Group (1 mentions) Monoclonal antibody against id1, by Santa Cruz Biotechnology (1 mentions) Bi2536, by Selleck Chemicals (1 mentions) Gapdh, by Proteintech (1 mentions) Anti flag m2 bead, by Merck Group (1 mentions) Sds loading buffer, by Beyotime (1 mentions) Agarose beads, by Santa Cruz Biotechnology (1 mentions) Co immunoprecipitation lysis buffer, by Beyotime (1 mentions) Anti ybx1 antibody, by Cell Signaling Technology (1 mentions) Anti ape1 antibody, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-PLK1 (2 citations) Anti-TM (2 citations)

4 protocols using anti plk1 antibody

Investigating T-cell Leukemia Pathways

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The human T-lineage ALL cell lines, Jurkat and MOLT4, were purchased from the cell bank at Peking Union Medical University and cultured in RPMI 1640 (Keygentech Biotechnology Co, Ltd, Jiangsu, China) supplemented with 10% fetal bovine serum (Gibco), penicillin (100 U/mL), and streptomycin (100 mg/mL).
USP1 inhibitor ML323 and PLK1 inhibitor BI2536 were purchased from Selleck Chemicals. MG132 was purchased from Sigma-Aldrich. Glycolysis inhibitor 2-deoxyglucose (2-DG) was purchased from MedChemExpress(MCE). The primary antibodies against USP1, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and Flag were purchased from Proteintech. Anti-PLK1 antibody was purchased from Proteintech and Abcam. Anti-LDHA antibody was purchased from Beyotime Biotechnology. Anti-ubiquitin, AKT, and p-AKT (473) antibodies were purchased from Cell Signaling Technology. Monoclonal antibody against ID1 was purchased from Santa Cruz Biotechnology. Anti-human CD45 antibody was purchased from GeneTech Co, Ltd (Shanghai, China). PerCP anti-human CD45 antibody was purchased from Beijing Quantobio Biotechnology Co Ltd and phycoerythrin rat anti-mouse CD45 antibody was from BD Biosciences.

Liu S., Xiang Y., Wang B., Gao C., Chen Z., Xie S., Wu J., Liu Y., Zhao X., Yang C., Yue Z., Wang L., Wen X., Zhang R., Zhang F., Xu H., Zhai X., Zheng H., Zhang H, & Qian M. (2023). USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis. Blood Advances, 7(13), 3099-3112.

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Immunohistochemical Analysis of PLK1 in KIRP

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Adjacent normal tissues and KIRP tissues were fixed in 4% paraformaldehyde and then embedded in paraffin and sectioned to a thickness of 6 μm. For immunohistochemical staining, sections were deparaffinized and rehydrated. Rehydrated sections were treated with Tris-EDTA buffer solution containing 10 mM Tris-Hcl and 1 mM EDTA to expose antigens and inactivate endogenous peroxidases. The sections were then boiled in a pressure cooker for 5 min. After being washed 3 times, sections were incubated with BSA for 30 min and subsequently incubated with anti-PLK1 antibody (1:200 dilution; proteintech, China) overnight at 4°C. The following day, sections were incubated at room temperature with a secondary antibody for 1 h, then developed with DAB staining solution for 30 s. After the nucleus was stained with hematoxylin, sections were dehydrated and mounted using neutral resins.

Gan L., Xiao Q., Zhou Y., Fu Y, & Tang M. (2023). Role of anoikis-related gene PLK1 in kidney renal papillary cell carcinoma: a bioinformatics analysis and preliminary verification on promoting proliferation and migration. Frontiers in Pharmacology, 14, 1211675.

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Immunoprecipitation of Protein Complexes

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Cells cultured in 10-cm plates of 95% confluency were lysed in co-immunoprecipitation lysis buffer (Beyotime), briefly sonicated and centrifuged for clarification. Proteins were quantified using a BCA assay kit, then incubated with either agarose beads (Santa Cruz Biotechnology) and the intended antibodies including anti-APE1 antibody (Santa Cruz Biotechnology), anti-YBX1 antibody (Cell Signaling Technology), anti-PLK1 antibody (Proteintech), and anti-PPP1R12A antibody (Proteintech) or IgG (Proteintech) or with Anti-flag M2 beads (Sigma-Aldrich) at 4 °C overnight. Beads were washed four times with co-immunoprecipitation buffer with rotation at 4 °C for 5 min each time, boiled with 5× SDS loading buffer (Beyotime), clarified by centrifugation and loaded onto an SDS-PAGE gel for WB analysis as described above. For detecting interacting proteins in the cytoplasm, the cytoplasmic fraction obtained by subcellular fractionation was used.

Mao S., Xie C., Liu Y., Zhao Y., Li M., Gao H., Xiao Y., Zou Y., Zheng Z., Gao Y., Xie J., Tian B., Wang L., Hua Y, & Xu H. (2024). Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) promotes stress granule formation via YBX1 phosphorylation in ovarian cancer. Cellular and Molecular Life Sciences: CMLS, 81(1), 113.

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Quantifying PLK1 Protein Expression

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The cells were transfected with PLK1 or control plasmid for 48h, and the protein was extracted. The protein extract was separated by SDS-PAGE and transferred to a PVDF membrane, which was then probed with an anti-PLK1 antibody (1:1000 dilution; proteintech, China). The antigen-antibody response was observed by a ChemiDoc system using a peroxide-coupled secondary antibody. ImageJ was used to quantify the intensity of the band.

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