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Pcagig venus

Manufactured by Thermo Fisher Scientific
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The PCAGIG-Venus is a laboratory instrument designed for the detection and analysis of various biological molecules. It utilizes a highly sensitive and precise method known as GIG (Gel Integrated Gel) technology to perform accurate and reliable measurements. The core function of the PCAGIG-Venus is to provide researchers and scientists with a reliable tool for their analytical needs in a wide range of applications.

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Lab products found in correlation

Shc001, by Merck Group (2 mentions) Cm3050, by Leica (2 mentions) Trcn0000330971, by Merck Group (2 mentions) F13082, by Thermo Fisher Scientific (2 mentions)

2 protocols using pcagig venus

1

Manipulating Neuronal Progenitors in Cerebral Organoids

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Organoids (specifically, the “ventricles” or progenitor zones) were electroporated with either a MeCP2 shRNA construct (validated sequence from Sigma; TRCN0000330971) or control (SHC001, Sigma), each co-injected with a Venus construct (pCAGIG-Venus; gift from Omar Durak and Prof. Li-Huei Tsai) at 8 weeks post-EB formation. Indicated plasmids were mixed at the following concentrations: shRNA (shMeCP2) / control (shControl) plasmids, 1 μg/ μl; pCAG-Venus plasmid, 0.5 μg/μl. Immediately after DNA injection into the organoid, four 50-ms electrical pulses (40V) were applied at 1-s intervals using a 5-mm electrode and an electroporator (EM830, BTX). After 7 days, organoids were fixed (4% PFA) and cryoprotected in 20% and 30% sucrose solutions, respectively, overnight. Fixed organoids were sliced on a cryostat (Leica, CM 3050 S) into 20 μM sections. For tracing migration of newly born neurons, cerebral organoids from Rett-WT2 and RTT-Mut2 cells were co-injected with pCAGIG-Venus and fluorescence beads (Molecular Probes; F13082) that mark the location of the injected ventricles.
Mellios N., Feldman D.A., Sheridan S.D., Ip J.P., Kwok S., Amoah S.K., Rosen B., Rodriguez B.A., Crawford B., Swaminathan R., Chou S., Li Y., Ziats M., Ernst C., Jaenisch R., Haggarty S.J, & Sur M. (2017). MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling. Molecular psychiatry, 23(4), 1051-1065.
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2

Manipulating Neuronal Progenitors in Cerebral Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organoids (specifically, the “ventricles” or progenitor zones) were electroporated with either a MeCP2 shRNA construct (validated sequence from Sigma; TRCN0000330971) or control (SHC001, Sigma), each co-injected with a Venus construct (pCAGIG-Venus; gift from Omar Durak and Prof. Li-Huei Tsai) at 8 weeks post-EB formation. Indicated plasmids were mixed at the following concentrations: shRNA (shMeCP2) / control (shControl) plasmids, 1 μg/ μl; pCAG-Venus plasmid, 0.5 μg/μl. Immediately after DNA injection into the organoid, four 50-ms electrical pulses (40V) were applied at 1-s intervals using a 5-mm electrode and an electroporator (EM830, BTX). After 7 days, organoids were fixed (4% PFA) and cryoprotected in 20% and 30% sucrose solutions, respectively, overnight. Fixed organoids were sliced on a cryostat (Leica, CM 3050 S) into 20 μM sections. For tracing migration of newly born neurons, cerebral organoids from Rett-WT2 and RTT-Mut2 cells were co-injected with pCAGIG-Venus and fluorescence beads (Molecular Probes; F13082) that mark the location of the injected ventricles.
Mellios N., Feldman D.A., Sheridan S.D., Ip J.P., Kwok S., Amoah S.K., Rosen B., Rodriguez B.A., Crawford B., Swaminathan R., Chou S., Li Y., Ziats M., Ernst C., Jaenisch R., Haggarty S.J, & Sur M. (2017). MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling. Molecular psychiatry, 23(4), 1051-1065.
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