Caspase 3 activities were measured by using colorimetric caspase 3 activity assay kit (Beyotime Biotech, China) according to the manufacturer's instruction and expressed as fold change. DNA fragment quantifications were performed with the DNA fragmentation kit (Takara, China) according to the manufacturer's protocol.
Dna fragmentation kit
Manufactured by Takara Bio
Sourced in China
The DNA fragmentation kit is a laboratory tool used for controlled fragmentation of DNA samples. It allows for the creation of DNA fragments of defined size ranges through a standardized mechanical shearing process. The kit provides the necessary components and protocols to reliably generate fragmented DNA suitable for downstream applications such as library preparation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Caspase 3 colorimetric activity assay kit, by Beyotime (1 mentions)
Gel doc, by Bio-Rad (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
Edu assay, by Abcam (1 mentions)
Sephadex g 25, by Merck Group (1 mentions)
Tac kit, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Giemsa, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
3 protocols using dna fragmentation kit
1
Caspase 3 and DNA Fragmentation Assay
2
DNA Fragmentation Analysis Protocol
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DNA fragmentation (DNA fragmentation kit-TaKaRa; 6137) was detected by agarose gel electrophoresis. The cell suspension remaining after Trypan blue (970 µL, 2 × 106/mL) was lysed in 5 mM Tris, 20 mM EDTA, and 0.5% Triton X-100 (pH 8.0) at 4°C for 1 hour and centrifuged at 13,000 rpm for 15 minutes. DNA was ethanol precipitated overnight at -20°C, further treated with proteinase K and RNAse, loaded on 1.0% agarose gels, and electrophoresis maintained at a constant voltage of 70 V for 2 hours. Fragmentation of DNA was visualized with ethidium bromide using the Bio-Rad gel doc.
3
Evaluating α-lactalbumin's Anti-Cancer Potential
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Materials used in this study are Human α-lactalbumin (Sigma), oleic acid (Sigma), G25 Sephadex (Sigma), ANS (Sigma), RPMI-1640 media (Gibco), antimycotic and antibacterial (Thermo fisher), FBS (Gibco), MTT assay (ab211091 MTT Cell Proliferation Assay Kit), Trypan blue (Thermo), Giemsa (Sigma), Hoechst dye (ab228551 Hoechst 33342), DNA Fragmentation kit (TaKaRa 6137) TAC kit (sigma), Annexin V-FITC assay (Milentyi), EdU Assay (Abcam), Survivin assay (Abcam).
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