Detection of apoptosis was performed using the Annexin V-APC Apoptosis Detection Kit II (BD Bioscience, San Jose, CA, USA) according to manufacturer’s protocol.
Annexin 5 apc apoptosis detection kit 2
Manufactured by BD
Sourced in United States
The Annexin V-APC Apoptosis Detection Kit II is a laboratory tool used for the detection and analysis of apoptosis, a programmed cell death process. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. The kit also includes the fluorescent dye 7-AAD to identify late apoptotic and necrotic cells. This kit provides a reliable method for the quantification of apoptosis through flow cytometry analysis.
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2 protocols using annexin 5 apc apoptosis detection kit 2
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Annexin V-APC Apoptosis Detection
2
BrdU Incorporation and Apoptosis Assay
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The BrdU incorporate assay was performed using BrdU-APC flow kit according to the manufacturer instruction (BD Pharmingen). CML mice were injected with 10mg/ml BrDU. 24 hrs post injection, bone marrow cells were extracted from CML mice and analyzed by flow cytometry. Apoptosis of bone marrow cells was measured by using the Annexin V-APC apoptosis detection kit II along with 7AAD staining for cell viability, according to the manufacturers instruction (BD Pharmingen. Differentially labeled cells were resolved using flow cytometry (BD FACS Calibur) and analyzed with Flowjo 5.2 software (Tree Star). Cells stained positive for Annexin V-APC and negative for 7AAD represented cells in early stage of apoptosis. 10,000 events from each sample were acquired to ensure adequate data.
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