Mouse specific elisa

Manufactured by Crystal Chem

Sourced in United States

Mouse-specific ELISA is a quantitative immunoassay used to detect and measure specific proteins or analytes in mouse samples. It involves the use of antibodies and color change to identify and quantify the target molecule.

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Lab products found in correlation

Onetouch ultra2, by LifeScan (1 mentions) Quantikine elisa kit for mouse rat igf 1 mg100, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse-specific ELISA kits (2 citations) ELISAs (3 citations)

2 protocols using mouse specific elisa

Measuring Fasting Glycemia and Insulinemia

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The fasting glycemia and insulinemia levels were measured in blood using the blood One Touch Ultra 2 (Lifescan, Inc., Milpitas, CA, USA) and the insulin ultrasensitive mouse-specific ELISA (Crystal Chem, Downers Grove, IL, USA) respectively.

Muñoz M.C., Burghi V., Miquet J.G., Giani J.F., Banegas R.D., Toblli J.E., Kopchick J.J., Bartke A, & Dominici F.P. (2014). DOWNREGULATION OF THE ANGIOTENSIN-CONVERTING ENZYME 2/ANGIOTENSIN-(1-7)/MAS RECEPTOR AXIS IN HEART AND KIDNEY OF TRANSGENIC MICE OVEREXPRESSING GROWTH HORMONE. The Journal of endocrinology, 221(2), 215-227.

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Quantification of Metabolic Biomarkers

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Blood samples were centrifuged at 2500 rpm for 20 min; and serum was collected and stored at −80 °C. Leptin levels were assayed using a mouse-specific ELISA (Crystal Chem, Downers Grove, IL). For sensitivity studies, Leptin was provided by Dr. Parlow (Harbor-UCLA Medical Center, Torrance, CA) through the National Hormone and Peptide Program. Mouse FGF21 and human FGF21 proteins were measured by ELISA (R & D Systems, MN). Analysis of intact FGF21 was performed by Lilly Research Laboratories by their in-house ELISA and also confirmed by a commercially available ELISA kit (F2131-K01; Eagle Biosciences, Nashua, NH). Plasma levels for insulin like growth factor−1 (IGF-1) were determined using Quantikine ELISA kit for mouse/rat IGF-1 (MG100; R&D Systems, MN).

Singhal G., Douris N., Fish A.J., Zhang X., Adams A.C., Flier J.S., Pissios P, & Maratos-Flier E. (2016). Fibroblast growth factor 21 has no direct role in regulating fertility in female mice. Molecular Metabolism, 5(8), 690-698.

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