Inactive Akt1, active Akt, active PDK1 and ATP/Mg2+ cocktail were purchased from Upstate Cell Signaling Solutions. Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2) was purchased from Calbiochem. 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phospho-L-serine (18:0,22:6-PS), 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (18:0, 22:6-PE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (16:0, 18:1-PC), and 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol-3,4,5-trisphosphate (18:0, 20:4-PIP3) were purchased from Avanti Polar Lipids. Sequencing grade modified trypsin was purchased from Promega. Anti-phospho-Akt (T308) (Cat#: 2965), anti-phospho-Akt (S473) (Cat#: 9271), anti-Akt (Cat#: 9272), anti-protein kinase C zeta (PKCζ)(Cat#: 9368), anti-phospho-PKCζ(T410) (Cat#: 9378), anti-phospho-PDK1(S241) (Cat#: 3061), anti-PDK1 (Cat#: 3062), and anti-GAPDH (Cat#: 2118) antibodies were purchased from Cell Signaling Technology. Anti-phospho-Akt (T34) (Cat#: 23509) was obtained from Abcam. A dilution of 1:1000 was used in western blotting for all of these antibodies. Insulin-like growth factor-1 (IGF-1) was purchased from PeproTech. PBS without Ca2+ was purchased from Quality Biological, Inc. Other reagents were purchased from Sigma or Quality Biological, Inc.
Anti phospho pdk1 s241
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho-PDK1(S241) is a primary antibody that recognizes the phosphorylated form of PDK1 at serine 241. PDK1 is a serine/threonine protein kinase that plays a central role in the PI3K/AKT signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti pdk1, by Cell Signaling Technology (3 mentions)
Anti phospho akt s473, by Cell Signaling Technology (2 mentions)
Anti akt, by Cell Signaling Technology (2 mentions)
Anti phospho pkc pan t514, by Cell Signaling Technology (2 mentions)
Trypsin, by Promega (2 mentions)
Insulin like growth factor 1 igf 1, by Thermo Fisher Scientific (1 mentions)
Anti phospho akt t308, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Sequencing grade modified trypsin, by Promega (1 mentions)
Anti vimentin antibody, by Santa Cruz Biotechnology (1 mentions)
Anti pkc pan, by Cell Signaling Technology (1 mentions)
Anti p38 map kinase, by Cell Signaling Technology (1 mentions)
Anti phospho hsp27, by Cell Signaling Technology (1 mentions)
Anti phospho atf2, by Cell Signaling Technology (1 mentions)
Anti phospho erk1 2 t202 y204, by Cell Signaling Technology (1 mentions)
Anti fak, by Cell Signaling Technology (1 mentions)
Anti phospho mek1 2 s217 221, by Cell Signaling Technology (1 mentions)
Anti pkc pan, by Merck Group (1 mentions)
Anti app, by Cell Signaling Technology (1 mentions)
3 protocols using anti phospho pdk1 s241
1
Akt and PDK1 Activation Assay
2
Signaling Pathways Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following reagents and materials were used: anti-GAPDH (Calbiochem), anti-MKK3, anti-MKK6, anti-phospho MKK3 (Ser189)/MKK6 (Ser207), anti-p38 MAP kinase, anti-phospho p38 MAP kinase (Thr180/Tyr 182), anti-phospho HSP27 (Ser82), anti-phospho ATF2 (Thr71), anti-PDK1, anti-phospho PDK1 (S241), anti-PKC-Pan, anti-phospho PKC-pan (T514), anti-Prohibitin 1 (Phb1), anti-Prohibitin 2 (Phb2), (Cell signaling). Anti-vimentin antibody was purchased from Santa Cruz biotechnology. Anti-phospho Phb1 (T258), and anti-phospho Phb1 (Y259) were purchased from Signalway Antibody. Electrophoresis reagents were purchased from Biorad and trypsin from Promega.
3
Comprehensive Signaling Pathway Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following reagents and materials were used. From Cell Signaling technology (Danvers, MA, USA): anti-APP (ref. 2450), anti-FAK (ref. 3285), anti-phospho-FAK (Y576/577) (ref. 3281), anti-Akt (ref. 4685), anti-phospho-Akt (S473) (ref. 4060), anti-PDK1 (ref. 3062), anti-phospho-PDK1 (S241) (ref. 3061), anti-phospho-PKC pan (T514) (ref. 9379), anti-p38 MAPK (ref. 9212), anti-phospho-p38 MAPK (T180/Y182) (ref. 9211), anti-MEK1/2 (ref. 9126), anti-phospho-MEK1/2 (S217/221) (ref. 9154), anti-ERK1/2 (ref. 9102), anti-phospho-ERK1/2 (T202/y204) (ref. 4370), anti-CREB (ref. 9104), anti-IκBα (ref. 4814S), anti-phospho-IκBα (S32) (ref. 2859), anti-NFκB p65 (ref. 8242), anti-phospho-NFκB p65 (ref. 3033S), anti-SEK1 (ref. 9152), and anti-phospho-SEK1 (S257/T261) (Ref. 9156). Anti-PKC-pan was from SigmaAldrich (St Louis, MO, USA) (ref. SAB4502356). Electrophoresis reagents were purchased from Bio-Rad (Hercules, CA, USA) and trypsin from Promega (Madison, WI, USA).
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