Anti e selectin

Manufactured by BD

Anti-E-selectin- is a laboratory reagent used for the detection and quantification of E-selectin, a cell adhesion molecule involved in various biological processes. It can be used for research and analytical applications.

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Lab products found in correlation

Anti e selectin clone 10e9, by BD (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions) Ve cadherin, by Santa Cruz Biotechnology (1 mentions) Goat anti claudin 5, by Santa Cruz Biotechnology (1 mentions) Mouse anti icam 1, by BD (1 mentions) Anti vcam 1, by BD (1 mentions)

Spelling variants of this product

E-selectin (10 citations) Phycoerythryin-Cy5-conjugated anti-E-selectin (2 citations) Anti–E-selectin (clone 10E9.6, (2 citations) Anti-E-selectin PE (2 citations)

2 protocols using anti e selectin

In Vivo Visualization of E-selectin Expression

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E-selectin vascular expression in the calvarial BM was imaged in vivo using anti–E-selectin (clone 10E9.6, BD Biosciences) conjugated to the fluorophore AF-647.SCID mice were engrafted with BCCs (0.1 × 10⁶ cells) 20 hours before imaging. Engrafted mice were injected with anti–E-selectin–AF-647 or rat IgG2a,κ–AF-647 isotype control (clone 35–95, BD Biosciences) at a dose of 1 mg/kg 18 hours before imaging. Mice were imaged, and montage images were generated as previously described.

Price T.T., Burness M.L., Sivan A., Warner M.J., Cheng R., Lee C.H., Olivere L., Comatas K., Magnani J., Lyerly H.K., Cheng Q., McCall C.M, & Sipkins D.A. (2016). Dormant breast cancer micrometastases reside in specific bone marrow niches that regulate their transit to and from bone. Science translational medicine, 8(340), 340ra73.

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Immunoassay Antibody Characterization Protocol

Cited 1 time

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Mouse anti-HRPII (2G12), a generous gift from Diane Taylor (University of Hawaii), was used at 1:100, goat anti-claudin-5 (from Santa Cruz; sc-17667) at 1:100, and VE-cadherin (Santa Cruz; sc-52751) at 1:100. Five micrograms of anti-HRPII was used for neutralization and was from Thermo Scientific (MA1-27094), and the isotype control (269) was generated as described previously (65 (link)). Rabbit anti-IL-1β (Rockland Immunochemical; 209-401-301) was used for neutralization. Rabbit anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase) (Abcam; ab37168) was used at 1:1,000. Mouse anti-ICAM-1, anti-VCAM-1, and anti-E-selectin (BD Biosciences; 555510, 555645, and 555648) were used at 1:500, 1:100, and 1:500 dilutions, respectively. Armenian hamster anti-IL-1β (Leinco) was used for in vivo neutralization at 300 µg/mouse.

Pal P., Daniels B.P., Oskman A., Diamond M.S., Klein R.S, & Goldberg D.E. (2016). Plasmodium falciparum Histidine-Rich Protein II Compromises Brain Endothelial Barriers and May Promote Cerebral Malaria Pathogenesis. mBio, 7(3), e00617-16.

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