Bone marrow cells were harvested as described in the Flow cytometry section. 106 bone marrow nucleated cells were plated into a 9.6cm2 glass bottom chamber slide (Lab-Tek II, Nunc) and cultured in MesenCult Proliferation medium for mouse cells (StemCell technologies) for 10 days. Cells were fixed in 4% paraformaldehyde, for 30 minutes at 4C° and counterstained with Wheat Germ Agglutinin Alexa Flour 488 conjugate (Invitrogen W11261) for 15 minutes. Representative images from at least three independent biological samples were shown in the figures.
Wheat germ agglutinin alexa flour 488 conjugate
Manufactured by Thermo Fisher Scientific
Wheat Germ Agglutinin Alexa Flour 488 conjugate is a fluorescently labeled lectin that binds to N-acetylglucosamine and sialic acid residues on cell surfaces. It is used as a tool in cell biology research to label and visualize specific cell types or structures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using wheat germ agglutinin alexa flour 488 conjugate
1
Bone Marrow Cell Culture and Imaging
2
Bone Marrow Cell Culture and Imaging
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!