Fam labelled sirna

Cholesterol and the lipids 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[lissamine rhodamine B sulfonyl] (DOPE-Rho) (all ≥99% purity according to thin-layer chromatography analysis) were purchased from Merck & Co., Inc (Kenilworth, N.J., USA). D-Lin-MC3-DMA (≥98% purity according to thin-layer chromatography analysis) was purchased from Assay Matrix Pty Ltd. (Ivanhoe North, VIC, Australia). Lincode siRNA targeting LINC01257 (Cat # 116437) was purchased from Horizon Discovery (Cambridge, UK). Non-targeting SCR siRNA, siRNA targeting ADARB2-AS1 and FAM-labelled siRNA were purchased from GenePharma (Shanghai, China).

Graphite powder was purchased from Acros. KMnO₄ (AR), NaNO₃ (AR), linear chain PEG amine (5000), branched polyethylenimine 10000 (BPEI 10000), folic acid, EDC and NHS, were purchased from Sigma-Aldrich. Doxorubicin was purchased from Sangon Biotech (Shanghai, China). Modified RPMI-1640 Medium was purchased from HyClone. Fetal bovine serum (FBS), penicillin-streptomycin, and trypsin were obtained from Gibco. Propidium iodide, LysoTracker, MitoTracker and Hoechst were purchased from Molecular Probes (USA). The Cell Counting Kit-8 (CCK-8) and Calcein AM were purchased from Dojindo (Japan). The β-actin antibody was purchased from GeneTex (USA). The dialysis bags and ultrafiltration tubes (molecular weight cutoff: 10-kDa and 30-kDa) were purchased from Millipore. The P-gp siRNA, the scrambled siRNA and FAM-labelled siRNA were supplied by GenePharma (Shanghai, China).