Reactions were run in flame-dried glassware under an atmosphere of argon or nitrogen. All solvents were dried and/or purified according to standard procedures prior to use. Melting points were determined with an Electrothermal Digital Melting Point Apparatus, Cole-Parmer ET0001/Version 1.0, and were uncorrected. Optical rotations were measured using a P3000 series polarimeter (Krüss Optronic GmbH, Hamburg, Germany). NMR spectra were recorded on Varian spectrometers (Varian Medical Systems, Inc., Palo Alto, CA, USA). 1H NMR spectra were recorded at 300 MHz or 600 MHz; 13C NMR spectra were recorded at 75 MHz or 150 MHz and were internally referenced to residual solvent peaks. Chemical shifts are reported in δ units, parts per million (ppm). Low resolution mass spectra were recorded on an LC-MSn Fleet mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) using MeOH as the solvent. High resolution mass spectrometry (HRMS) spectra were recorded on a UPLC-MSn Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Flash column chromatography (FCC) was performed on silica gel 60 (230–400 mesh, Merck KGaA, Darmstadt, Germany) and thin layer chromatography (TLC) on precoated glass plates 60 F254 (0.2 mm, Merck KGaA, Darmstadt, Germany). Spots were visualized using UV light at 254 nm and phosphomolybdic acid stain (PMA, 10% in absolute ethanol).
Varian spectrometers
Manufactured by Agilent Technologies
Sourced in United States
Varian spectrometers are analytical instruments used for the detection and identification of chemical compounds. They are designed to measure the absorption or emission of electromagnetic radiation by samples, providing information about their molecular structure and composition.
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Lab products found in correlation
6 protocols using varian spectrometers
1
General Organic Synthesis Protocol
2
NMR Analysis of DNA Samples
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Nuclear magnetic resonance (NMR) experiments were conducted on 500 and 800 MHz Varian spectrometers at 25°C. The concentration of DNA samples was typically ∼0.1 mM.
3
NMR Spectroscopy Protocol for Biomolecular Characterization
4
NMR Characterization of Biomolecular Samples
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Experiments were performed on 500 MHz, 750 MHz and 800 MHz Varian spectrometers. Resonances were assigned unambiguously using samples with site-specific low-enrichment 15N or 15N, 13C labeling and through-bond correlations at natural abundance. Standard 2D NMR experimental spectra including NOESY, TOCSY and COSY, were collected at 25 °C to obtain the complete proton resonance assignments. The NMR experiments for samples in water solution were performed with Watergate or Jump-and-Return water suppression techniques. The variable temperature spectra were recorded on 500 MHz Varian spectrometer from 5 °C to 50 °C. All spectra were processed by the program NMRPipe. Spectral assignments were also carried out and supported by COSY, TOCSY and NOESY spectra. Peak assignments were achieved using the software Sparky.
5
NMR Characterization of Troponin C Complexes
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The NMR samples consisted of 0.3–0.8 mM cTnC(C35S)-i9, cNTnC-i9, cChimera-i9, or cNTnC(C35S)-i9 in 500 or 600 μL of 100 mM KCl, 10 mM imidazole or imidazole-d4, 2 mM CaCl2, and 0.25 mM 2,2-dimethyl-2-silapentane-5-sulfonate-d6 sodium salt (DSS-d6) or trifluoroacetic acid (TFA) as internal reference, at pH 6.9 (NMR buffer). The NMR experiments were acquired in 500, 600, or 800 MHz Varian spectrometers at 30 °C. All one-dimensional experiments were processed with VnmrJ v 3.2, all the multidimensional spectra were processed with NMRPipe [54] (link) and analyzed with NMRViewJ [55] (link). The assignment of free i9 in DMSO was done based on examination of the 1H NMR spectra acquired throughout the synthesis, and on previous assignment of the levosimendan analog dfbp-o [25] (link). The assignment of i9 in cChimera-i9 was achieved using the 13C, 15N filtered noesy, 13C, 15N filtered tocsy, and 1H, 19F HMQC spectra (Supporting Figure 3 and Supporting Table 2). Assignment of cChimera in cChimera-i9 was done by using typical 2d and 3d NMR experiments 1H, 15N- and 1H, 13C-HSQC, HNCACB, CBCA(CO)NH, HNHA, HCCONH, and CCONH detailed in Supporting Table 2.
6
NMR Spectroscopy of Oligonucleotides
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The 1D 1H NMR experiments were performed on 750 MHz and 800 MHz Varian spectrometers. Watergate or Jump-and-Return water suppression techniques were employed for samples in water solution. NMRPipe was used to process NMR spetra. For the HDX exchange experiments, the oligonucleotides samples were annealed and lyophilized. The lyophilized oligonucleotides were resuspended in 99% D2O before NMR measurements.
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