NS-398 was purchased from Alexis Deutschland GmbH (Grünberg, Germany). Aprotinin, Δ-glycerophosphate, ethylenediaminetetraacetic acid (EDTA), leupeptin, lovastatin lactone, luminal, mevastatin, p-coumaric acid, phenylmethylsulfonyl fluoride (PMSF), (R)-mevalonic acid lithium salt, sodium molybdate and sodium orthovanadate were obtained from Sigma-Aldrich (Taufkirchen, Germany). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was from Ferak (Berlin, Germany). Dimethyl sulfoxide (DMSO), dithiothreitol (DTT), glycerol, p-nitrophenylphosphate, sodium chloride, sodium dodecylsulfate (SDS) and sodium fluoride were from AppliChem (Darmstadt, Germany) and GW9662 and Nonidet® P-40 from Enzo Life Sciences (Lörrach, Germany). Lovastatin hydroxy acid, sodium salt, was provided from Toronto Research Chemical (Toronto, Canada) and Triton® X-100, acetonitrile (LC-MS grade) and trifluoroacetic acid (analytical grade) from Roth (Karlsruhe, Germany). Penicillin-streptomycin was from Invitrogen (Darmstadt, Germany). Dulbecco's Modified Eagle's medium (DMEM) with 4 mM L-glutamine and 4.5 g/L glucose was from Lonza (Cologne, Germany). Phosphate-buffered saline (PBS) and fetal calf serum (FCS) were obtained from PAN Biotech (Aidenbach, Germany).
Sodium fluoride
Manufactured by AppliChem
Sourced in Germany
Sodium fluoride is a white crystalline solid chemical compound with the formula NaF. It is used in various laboratory applications as a source of fluoride ions.
Automatically generated - may contain errors
Lab products found in correlation
Leupeptin, by Merck Group (2 mentions)
Sodium orthovanadate, by Merck Group (2 mentions)
Sodium molybdate, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by AppliChem (1 mentions)
Trifluoroacetic acid, by Carl Roth (1 mentions)
Glycerol, by AppliChem (1 mentions)
Luminal, by Merck Group (1 mentions)
P nitrophenyl phosphate, by AppliChem (1 mentions)
Gw9662, by Enzo Life Sciences (1 mentions)
Dulbecco s modified eagle s medium dmem with, by Lonza (1 mentions)
Phosphate buffered saline (pbs), by PAN Biotech (1 mentions)
Mevastatin, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
L glutamine, by Lonza (1 mentions)
Fetal calf serum (fcs), by PAN Biotech (1 mentions)
Sodium chloride (nacl), by AppliChem (1 mentions)
Okadaic acid, by Cayman Chemical (1 mentions)
Sodium pyrophosphate, by Merck Group (1 mentions)
2 protocols using sodium fluoride
1
Reagent Procurement for Cell Culture Assays
2
Protein Extraction and Detection Protocol
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Cultured cells were lysed in NOP buffer (10 mm Tris-HCl pH 7.4, 150 mm NaCl, 1 mm MgCl 2 , 1 mm CaCl 2 , 1% nonidet P40, 0.2% Triton X-100) supplemented with protease inhibitors [1 μm aprotinin (Carl Roth, Karlsruhe, Germany), 1 mm pefabloc (Serva, Heidelberg, Germany), 2.5 μm soy bean trypsin inhibitor, 2.1 μm leupeptin (both Sigma-Aldrich)] and with phosphatase inhibitors [10 mm sodium fluoride (AppliChem, Darmstadt, Germany), 1 mm sodium orthovanadate, 25 mm β-glycerophosphate and 10 mm sodium pyrophosphate (all Sigma-Aldrich), 750 nm okadaic acid (Cayman Chemical Company, Ann Arbor, MI, USA)]. Fifty micrograms of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane (Protran BA85, GE Healthcare, Munich, Germany). Proteins were visualized using the Odyssey Sa Infrared Imaging System (LI-COR Bioscience, Lincoln, NE, USA) or enhanced chemiluminescence (ECL, Ther-moFisher Scientific). The quantification of the bands' intensities was calculated using the software ImageStudio (LI-COR, version 5.02).
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