Details of the paradigm used in the current study are reported in Heun-Johnson and Levitt (2016) (link), based on methods reported in Rice et al. (2008) (link). Briefly, ELS is induced by inserting a wire mesh (#RWF75JMV, Allentown Inc., NJ) into the cage on postnatal day (P)2, and providing the dams with two-thirds (1.8 g) of a nestlet square (Ancare Corp, NY). Control cages lacked the wire mesh insert, and contained standard amount of bedding and one nestlet square. All litters were culled to three males and two females on P2. ELS and Control litters were placed into a fresh control cage environment on P9. Additional cage changes were carried out on P16, and at time of weaning (P21).
Nestlet square
Manufactured by Ancare
The Nestlet square is a laboratory equipment item. It serves as a nesting material for rodents in a laboratory environment.
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Lab products found in correlation
3 protocols using nestlet square
1
Early Life Stress Induction Protocol
2
Nesting Behavior Assessment in Rodents
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Animals were placed in individual cages overnight (~14 hrs) with a ~5 cm cotton Nestlet™ square (Ancare, Bellmore, NY). In the morning, nest building was assessed by applying the nest complexity scoring matrix(11 (link)). Nesting scores were based on a 0–5 scale with 0.5 increments.
3
BALB/c Mouse Husbandry for Biomedical Research
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Female BALB/c mice (N = 212) were purchased from Charles River (strain code 028). The mice used in these experiments were 6–10 weeks of age and weighed 15–22 g. All mice received irradiated food (PicoLab Rodent Diet 5053, LabDiet) and water ad libitum. All mice were housed in groups (n = 5) in sanitized solid-bottom individually ventilated cages (Sealsafe Plus GM500, Techniplast) on sterile paper bedding (Alpha-dri, Shepherd Specialty Paper) and were provided with environmental enrichment including a plastic Mouse Igloo with saucer wheel (BioServ) and a single Nestlet square (Ancare). Animals were maintained under a 12:12 hour light/dark cycle, with 10–15 air changes per hour, relative humidity of 30–70%, and room temperature between 68–79ºF (20–26.1ºC). From entry into the facility, all mice were housed under Animal Biosafety Level 2 (ABSL-2) conditions, where they acclimated to the facility for at least 5 days. Animals were identified using cage cards and tail marks with a permanent marker. Prior to the start of the study, all animals were monitored twice daily during routine animal health rounds.
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