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P24 fitc clone kc57

Manufactured by Beckman Coulter
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The P24 FITC (clone KC57) is a flow cytometry reagent used to detect the p24 antigen of the human immunodeficiency virus (HIV). It is a fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody that binds to the p24 capsid protein of HIV. This reagent is used for the identification and enumeration of HIV-infected cells in clinical and research applications.

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Lab products found in correlation

Cd8 pb clone rpa t8, by BD (1 mentions) Live dead blue dye, by Thermo Fisher Scientific (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd4 apc h7 clone rpa t4, by BD (1 mentions) Cd4 bv605 clone rpa t4, by BD (1 mentions) Cd3 apc cy7 clone sk7, by BioLegend (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Cd4 v450, by BD (1 mentions) Cd8 percp, by BD (1 mentions) Cd3 v500, by BD (1 mentions)

Close spelling variants of this product

Anti-p24-FITC (clone KC57, P24 KC57-FITC KC57-FITC

3 protocols using p24 fitc clone kc57

1

Comprehensive Flow Cytometry for Immune Cell Analysis

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Flow cytometry was performed on a LSRFortessa Flow Cytometer (BD Biosciences) and the data were analyzed using FlowJo software. The following antibodies and regents were used: CD8 PB (clone RPA-T8) was from BD Biosciences; CD45RA PE-Cy7 (clone H2100), CCR7 APC (clone 3D12), CD38 PE-Cy7 (clone HIT2) were from eBioscience; CD3 PE/Dazzle594 (clone UCHT1), CD25 PE (clone BC96), HLA-DR ECD (clone L243), T-bet PE (clone 4B10) were from BioLegend; p24 FITC (clone KC57) was from Beckman; Live/Dead™ blue dye (Invitrogen) was used for distinguishing live and dead cells. Measurements of cytokines in cell culture medium were performed with BD™ Cytometric Bead Array Th1/2/17 kit according to the manufacturer’s protocol.
Zhu L., Qiu C., Dai L., Zhang L., Feng M., Yang Y., Qiu C., Zhang A., Huang J., Wang Y., Wan Y., Zhao C., Wu H., Lyu J., Zhang X, & Xu J. (2021). Hsa-miR-31 Governs T-Cell Homeostasis in HIV Protection via IFN-γ-Stat1-T-Bet Axis. Frontiers in Immunology, 12, 771279.
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2

Multicolor Flow Cytometry of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated from fresh heparinized blood via Ficoll–Hypaque density gradient centrifugation (Pharmacia, Uppsala, Sweden). Multicolor flow cytometry with fluorescent conjugated antibodies obtained from BioLegend (San Diego, USA) were as follows: CD3-APC-Cy7 (clone SK7), CD8-Percp/Cyanine5.5 (clone SK1), CD56-BV421 (clone HCD56), CCR5-FITC (clone J418F1), and CXCR4-APC (clone RG5). CD4-APC-H7 (clone RPA-T4) and CD4-BV605 (clone RPA-T4) were obtained from BD Biosciences (New Jersey, USA). PBMCs were first stained at 4°C for 30 min with fluorescent antibodies under dark conditions. Cells were then permeabilized using a Cytofix/Cytoperm Kit (BD Bioscience) and stained with p24-PE (clone KC57) or p24-FITC (clone KC57) from Beckman Coulter (Eurocenter S.A, California, USA). The cells were fixed in 0.5% formaldehyde and analyzed using a FACSCantoTM flow cytometer (BD Biosciences). The data were analyzed using the FlowJo software (TreeStar).
Gao L., Jiao Y.M., Ma P., Sun L., Zhao H., Guo A.L., Fan X., Zhang C., Song J.W., Zhang J.Y., Lu F, & Wang F.S. (2022). Characterization and distribution of HIV-infected cells in semen. Emerging Microbes & Infections, 11(1), 860-872.
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3

Multicolor Flow Cytometry for HIV T-Cell Immune Profiling

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In this study, we used three fluorochrome-labeled surface-marker antibodies: CD3 V500 (1,032,587) for CD3+ T cells, CD4 V450 (10,556,120) for CD4+ T cells, and CD8 PerCP (1,035,212) for CD8+ T cells from BD Bioscience. We also used two clones of HIV1 p24 monoclonal antibodies: p24 FITC (clone KC57) from Beckman Coulter (6,604,665) and p24 APC (clone 28B7) from MediMabs (MM-0289-APC). We also used antibodies of the two ICMs: PD1 PE (clone eBioJ105 (J105) from Invitrogen (2–2799-42), and TIM3 PE-cy7 (clone F38-2E2) from Thermo Fisher Scientific, eBioscience (15,518,536).
Tessema B., Boldt A., König B., Maier M, & Sack U. (2022). Flow-Cytometry Intracellular Detection and Quantification of HIV1 p24 Antigen and Immunocheckpoint Molecules in T Cells among HIV/AIDS Patients. HIV/AIDS (Auckland, N.Z.), 14, 365-379.
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