Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (San Francisco, California, USA). Primary anti-bodies: rabbit anti-human S100A9 was bought from ABCAM (MA, USA). Rabbit anti-human total ERK1/2 MAPK, rabbit anti-human total p38 MAPK, rabbit anti-human total p50 NF-κB, rabbit anti-human total p65 NF-κB and antibodies against phospho-ERK1/2 MAPK, phospho-p38 MAPK, phospho-p50 NF-κB, phospho-65 NF-κB were bought from Cell Signaling Technology (Boston, Massachusetts, USA). Rabbit anti-human p21 and p27 were purchased from Anbo Biotechnology (San Francisco, California, USA). Rabbit anti-human Ki67 nuclear antigen, mouse anti-human PCNA and mouse anti-human glyceraldehydes-3-phosphate dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology (San Francisco, California, USA). Horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies were purchased from Zhong Shan Golden Bridge Biotechnology (Beijing, China).
Mouse anti human pcna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Mouse anti-human PCNA is a primary antibody that binds to the human Proliferating Cell Nuclear Antigen (PCNA) protein. PCNA is a key regulator of DNA replication and repair processes in eukaryotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rabbit anti human ki67 nuclear antigen, by Santa Cruz Biotechnology (2 mentions)
Mouse anti human glyceraldehydes 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (2 mentions)
Horseradish peroxidase conjugated goat anti rabbit and goat anti mouse secondary antibodies, by Zhongshan Golden Bridge Biotechnology (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rabbit anti human s100a9, by Abcam (1 mentions)
Phospho p38 mapk, by Cell Signaling Technology (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Molecular imager chemidox xrs imaging system, by Bio-Rad (1 mentions)
Mouse anti human β actin, by Merck Group (1 mentions)
Rabbit anti human rela, by Cell Signaling Technology (1 mentions)
Mouse anti human ikkε, by Santa Cruz Biotechnology (1 mentions)
Mouse anti human cyclin d1, by Santa Cruz Biotechnology (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mouse anti human β actin, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
3 protocols using mouse anti human pcna
1
Signaling Pathway Profiling in Cell Culture
2
Western Blot Analysis of Signaling Proteins
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Protein lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. After being blocked with primary and secondary HRP-conjugated antibodies in 5% nonfat milk or bovine serum albumin, immunoreactive proteins were detected with enhanced chemiluminescence reagents (Thermo Fisher Scientific). Blots were scanned by Molecular Imager ChemiDox XRS+ Imaging System with Image Lab software (Bio-Rad Laboratories). The following antibodies were used: rabbit anti–human RELA, rabbit anti–human FOS, rabbit anti–human p-RELA, mouse anti–human IκBα, rabbit anti–human p-IκBα, and mouse anti–human IKKα (Cell Signaling Technology); mouse anti–human IKKε, mouse anti–human PCNA, and mouse anti–human cyclin D1 (Santa Cruz Biotechnology, Inc.); mouse anti–human β-actin (Sigma-Aldrich); and goat anti–rabbit or goat anti–mouse IgG (Jackson ImmunoResearch Laboratories, Inc.). Densitometry of specific Western blot bands was analyzed using ImageJ version 1.48 software (National Institutes of Health) and the intensity values were normalized against the β-actin loading control. Experiments were repeated independently at least three times.
3
Investigating RSK2 Signaling Pathways
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Reagents. Fetal bovine serum (FBS) and DMEM were purchased from Gibco (San Francisco, CA, USA). Primary antibodies: rabbit anti-human RSK2 was from Bioworld (USA). Rabbit anti-human total c-Fos, rabbit anti-human total AKT, rabbit anti-human total mTOR, and phosphorylated ERK, phosphorylated AKT, phosphorylated mTOR were from Cell Signaling Technology (Boston, MA, USA). Rabbit antihuman Bax, Bcl2, caspase-3, rabbit anti-human Ki67 nuclear antigen, mouse anti-human PCNA, mouse anti-human β-actin and mouse anti-human glyceraldehydes-3-phosphate dehydrogenase (GAPDH) were from Santa Cruz Biotechnology (San Francisco, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies were from Zhong Shan Golden Bridge Biotechnology (Beijing, China).
shRNA. The RSK2 shRNA were designed and synthesized by Genechem Co. Ltd. (Shanghai, China) and the sequences targeting RSK2 were 5'-TGCCACAATACCAACTAAA-3'. A non-specific scrambled shRNA with a sequence of 5'-TTCTC CGAACGTGTCACGT-3' was used as a negative control.
shRNA. The RSK2 shRNA were designed and synthesized by Genechem Co. Ltd. (Shanghai, China) and the sequences targeting RSK2 were 5'-TGCCACAATACCAACTAAA-3'. A non-specific scrambled shRNA with a sequence of 5'-TTCTC CGAACGTGTCACGT-3' was used as a negative control.
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