Elan 6100 perkin elmer spectrometer

Manufactured by PerkinElmer

The ELAN 6100 Perkin Elmer Spectrometer is an inductively coupled plasma mass spectrometer (ICP-MS) designed for multi-element analysis. It is capable of detecting and quantifying a wide range of elements in various sample types, including environmental, geological, and biological samples. The ELAN 6100 provides high sensitivity, accuracy, and precision in elemental analysis.

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Lab products found in correlation

Certipur, by Merck Group (1 mentions)

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ELAN 6100 Spectrometers

2 protocols using elan 6100 perkin elmer spectrometer

Gadolinium Quantification in Tissues

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ICP‐MS analysis of Gd (¹⁵⁷Gd) content was performed on an ELAN 6100 Perkin Elmer Spectrometer (Waltham, MA) at Bracco Research Centre. Internal standardization was performed using ¹⁵³Eu. The calibration blanks, calibration standards, and control standard solutions for each analytical sequence were prepared in 2% nitric acid by dilution of a gadolinium oxide (Gd₂O₃) standard solution (1000 mg/mL in 2% HNO₃, Certipur, Merck).
The limit of quantitation (LOQ) for gadolinium was 0.1 nmol/mL for blood, 0.1 nmol/g for cerebrum/cerebellum, 0.5 nmol/g for femur, 1 nmol/g for liver and skin, and 1.7 nmol/g for kidney. The LOQ for each tissue was verified for accuracy and precision by spiking in triplicate explanted blank organs with the corresponding amounts of gadolinium and determining the respective percent recoveries.

Bussi S., Coppo A., Botteron C., Fraimbault V., Fanizzi A., De Laurentiis E., Colombo Serra S., Kirchin M.A., Tedoldi F, & Maisano F. (2017). Differences in gadolinium retention after repeated injections of macrocyclic MR contrast agents to rats. Journal of Magnetic Resonance Imaging, 47(3), 746-752.

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Quantifying Tf and HSA in Biological Samples

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The Tf and HSA concentrations in the studied systems were determined using human Tf or HSA ELISA kits, respectively. Standard curves were prepared with the supplied standards with concentration ranging from 9.375 to 600 ng/mL for Tf and from 6.25 to 200 ng/mL for HSA. The tested samples were diluted appropriately in a sample diluent buffer and the tests were performed according to the manufacturer’s protocol. ELISA 96-well plates were read using Tecan Infinite 200 Reader plate at 450 nm for both Tf and HSA. The total protein concentration in human plasma was measured by Bradford method.
The total Ru content in samples was measured by the application of ICP-MS using ELAN 6100 Perkin Elmer spectrometer. Prior to the determination of Ru content, 100 μL of the samples was mineralized using 500 μl of Ultrapure concentrated nitric acid and then diluted with water.
The experiments were carried out at least in triplicate, unless stated otherwise.

Śpiewak K, & Brindell M. (2015). Impact of low- and high-molecular-mass components of human serum on NAMI-A binding to transferrin. Journal of Biological Inorganic Chemistry, 20(4), 695-703.

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