Light chain 3

Manufactured by Cell Signaling Technology

Sourced in United States

Light chain 3 (LC3) is a protein involved in the process of autophagy, which is the degradation and recycling of cellular components. LC3 plays a crucial role in the formation of autophagosomes, the double-membrane vesicles that engulf the cargo to be degraded. As a key component of the autophagy machinery, LC3 is widely used as a marker to study and monitor autophagic processes.

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Lab products found in correlation

Matrigel basement matrix, by BD (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) Chloroquine cq, by Merck Group (1 mentions) 3 methyladenine 3 ma, by Merck Group (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Meloxicam, by Merck Group (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Hif 2α, by Novus Biologicals (1 mentions) Hif 1α, by Abcam (1 mentions) Parkin, by Abcam (1 mentions) Ecl reagent, by Merck Group (1 mentions) Sqstm1 p62, by Abcam (1 mentions) Actin hrp, by Santa Cruz Biotechnology (1 mentions) Superoxide dismutase 2 sod2, by Santa Cruz Biotechnology (1 mentions) Pink1, by Abcam (1 mentions) Iblot system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-light chain 3 (anti-LC3) Anti-light chain 3 (LC3), Anti-microtubule-associated protein light chain 3 (LC3) Microtubule-associated protein light chain 3 (LC3; Microtubule-associated protein-1 light-chain 3 (LC3)

3 protocols using light chain 3

Investigating Signaling Pathways in Cancer

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Antibodies (Abs) against MMP-1, MMP-2, COX-2, myeloid cell leukemia-1 (Mcl-1), Bax, Fas, Fas-L, AKT, phosphorylated AKT (p-AKT) (Ser473), survivin, Beclin-1 and light chain 3 (LC3) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Abs against E-cadherin and EP2 were from Abcam (Hong Kong, China). PGE2 protein, recombinant human MMP-2 protein (rh-MMP-2) and meloxicam dissolved in dimethyl sulfoxide (DMSO) were purchased from Merck Millipore (Merck Millipore, Darmstadt, Germany). Matrigel basement matrix (10 mg/ml) was purchased from BD Biosciences (San Jose, CA, USA), MK-2206 (an AKT inhibitor) from Jinan Trio Pharmatech Co., Ltd. (Jinan, China), and two autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ) from Sigma-Aldrich (Shanghai, China).

Dong X., Li R., Xiu P., Dong X., Xu Z., Zhai B., Liu F., Jiang H., Sun X., Li J, & Qiao H. (2014). Meloxicam Executes Its Antitumor Effects against Hepatocellular Carcinoma in COX-2- Dependent and -Independent Pathways. PLoS ONE, 9(3), e92864.

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Western Blot Profiling of Hypoxia Markers

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Protein lysates (30 μg protein per sample) were loaded and separated on sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and then transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was blocked with 5% nonfat milk and incubated with primary antibodies and corresponding second antibodies. Following primary antibodies were utilized in this study: HIF-1α (mouse monoclonal 1:500; Abcam, Cambridge, UK), HIF-2α (rabbit monoclonal 1:500; Novus, Littleton, CO), PHD2 (rabbit monoclonal 1:1500; Novus, Littleton, CO), Bcl-xL (rabbit monoclonal 1:1,000; Cell Signaling Technology, Danvers, MA), Bax (rabbit monoclonal 1:1,000; Cell Signaling Technology, Danvers, MA) or Light chain 3 (LC-3, rabbit monoclonal 1:1,000; Cell Signaling Technology, Danvers, MA).

Fang Y., Zhang H., Zhong Y, & Ding X. (2016). Prolyl hydroxylase 2 (PHD2) inhibition protects human renal epithelial cells and mice kidney from hypoxia injury. Oncotarget, 7(34), 54317-54328.

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Western Blot Analysis of Mitophagy Markers

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The cell pellet was lysed in an EzRipa buffer (ATTO, Tokyo, Japan). Lysates were collected by centrifugation at 13,000 rpm for 20 min. Protein concentration was measured using the bicinchoninic acid (BCA) method (Pierce, Waltham, MA, USA). Equal amounts of protein were separated on SDS-PAGE gels and transferred to membranes using an iBlot system (Life Technologies). Membranes were blocked with 5% skim milk in TTBS at room temperature for 1 hr. They were immunoblotted with specific primary antibodies Superoxide Dismutase 2 (SOD2, 1:500) and Actin-HRP (1:2000) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Light chain 3 (LC3, 1:1000) antibody was purchased from Cell Signaling (Danvers, MA, USA). PINK1 (1:2000), Parkin (1:2000), and p62/SQSTM1 (1:10000) antibodies were purchased from Abcam (Cambridge, MA, USA). Blots were performed with the corresponding anti-rabbit, antigoat, or anti-mouse IgGs conjugated with horseradish peroxidase (AbFrontier, Seoul, Korea) and detected using the ECL reagent (EMD Millipore, Darmstadt, Germany).

Lee A.Y., Jang Y., Hong S.H., Chang S.H., Park S., Kim S., Kang K.S., Kim J.E, & Cho M.H. (2017). Ephedrine-induced mitophagy via oxidative stress in human hepatic stellate cells. The Journal of toxicological sciences, 42(4).

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