Column chromatographic procedures were performed using silica gel (80–120 mesh and 200–300 mesh, Qingdao Marine Chemical Co. Ltd., Qingdao, China) and Sephadex™ LH-20 gel (40–70 μm; Merck, Darmstadt, Germany), whereas precoated silica gel (GF254, Qingdao Marine Chemical Co. Ltd., Qingdao) plates were used for TLC analyses. Spots were visualized by heating silica gel plates sprayed with 10% H2SO4 in EtOH. UV spectra were recorded using a Waters UV-2401A spectrophotometer equipped with a DAD and a cell of 1 cm pathlength. Methanolic samples were scanned from 190 to 400 nm in 1 nm steps. Semipreparative HPLC was performed on an Agilent 1120 apparatus equipped with a UV detector and a reversed-phase C18 column (5 μm, 10 × 250 mm, Welch Ultimate XB-C18). 1D (1H, 13C) spectra of all compounds were recorded on Bruker AM-600, AM-500, and AM-400 NMR spectrometers (Bruker, Karlsruhe, Germany), with TMS as the internal reference. |Enzymatic activity experiments were performed using SpectraMax i3x (Molecular Devices, Austria).
Am 600
Manufactured by Bruker
Sourced in Germany
The AM-600 is a nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It is designed to perform high-resolution analysis of chemical samples. The core function of the AM-600 is to provide precise measurement and characterization of molecular structures and compositions.
Automatically generated - may contain errors
Lab products found in correlation
Am 500, by Bruker (2 mentions)
Sephadex lh 20 gel, by Merck Group (1 mentions)
Uv 2401a spectrophotometer, by Waters Corporation (1 mentions)
Ultimate xb c18, by Hill-Rom (1 mentions)
Spectramax i3x, by Molecular Devices (1 mentions)
Zncl2, by Thermo Fisher Scientific (1 mentions)
Cocl2 6h2o, by Thermo Fisher Scientific (1 mentions)
Cu oac 2 h2o, by Thermo Fisher Scientific (1 mentions)
Co oac 2 4h2o, by Thermo Fisher Scientific (1 mentions)
Cucl2 2h2o, by Thermo Fisher Scientific (1 mentions)
Ni no3 2 6h2o, by Thermo Fisher Scientific (1 mentions)
Am 300, by Bruker (1 mentions)
Type 257 spectrometer, by PerkinElmer (1 mentions)
Delta prep, by Waters Corporation (1 mentions)
4 protocols using am 600
1
Chromatographic Purification and NMR Analysis
2
NMR Analysis of HPP-1 in D2O
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Twenty milligrams of HPP-1 were dissolved in 1.0 mL of D2O and placed in a nuclear magnetic tube. 1H-NMR and 13C-NMR analyses were conducted using a Bruker AM-600 nuclear magnetic resonance instrument.
3
Synthesis and Characterization of Chiral Alcohols
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d -Valinol, d -phenylglycenol, d -leucinol, Ni (NO3)2·6H2O, Co(OAc)2.4H2O, Cu (OAc) 2.H2O, CuCl2·2H2O, CoCl2·6H2O, ZnCl2, and Cu(ClO4)2·6H2O were purchased from Acros. 1HNMR spectra were obtained using a Bruker AM-300 spectrometer. 1H and 13C NMR spectra were recorded using Bruker AM-500 and Bruker AM-600 spectrometers. Chemical shifts were reported in ppm (δ) with the solvent relative to tetramethylsilane (TMS) used as the internal standard (residual CHCl3, δH 7.26 ppm; CDCl3, C, 77 ppm). The following abbreviations were used to designate multiplicities: s = singlet, d = doublet, t = triplet, m = multiplet. Infrared spectra were recorded on a Mattson Galaxy Series FTIR 3000 spectrometer; peaks are reported in cm−1. Elemental analyses were performed on an Elemental Analyser AE-3000. The crystal structure was determined by a Gemini S Ultra diffractometer.
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4
Analytical characterization of compounds
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Optical rotations were measured at 25 °C on a Polaar 32 polarimeter. UV spectra were recorded on a Lightwave II + WPA 7126 V. 1.6.1 spectrophotometer. ECD spectra were measured at 25 °C on a JASCO J-810 spectropolarimeter. IR spectra were recorded with a PerkinElmer type 257 spectrometer. The NMR spectra were recorded on a Bruker AM-600 (600 MHz) NMR spectrometer equipped with a TCI 5 mm cryoprobe using CDCl as a solvent. The solvent signal was used as a reference. Sunfire preparative C columns (150 × 19 mm, i.d. 5 µm and 150 × 30 mm, i.d. 5 µm; Waters) were used for preparative HPLC separations using a Waters Delta Prep equipped with a binary pump (Waters 2525) and a UV-visible diode array detector (190-600 nm, Waters 2996). Silica 330 g, 120 g, and 24 g Grace cartridges were used for flash chromatography using an Armen Instrument spot liquid chromatography flash apparatus. Chemicals and solvents were purchased from Sigma-Aldrich. Create PDF in your applications with the Pdfcrowd HTML to PDF API PDFCROWD
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