Hitrap mabselect prisma protein a column

Manufactured by Cytiva

The HiTrap MabSelect PrismA protein A column is a chromatography column designed for the purification of monoclonal antibodies (mAbs) from cell culture supernatants or other biological samples. The column contains a recombinant protein A ligand immobilized on a highly cross-linked agarose matrix, which selectively binds to the Fc region of mAbs, allowing their capture and purification.

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Lab products found in correlation

Expi293f, by Thermo Fisher Scientific (2 mentions) Akta pure 25m fast protein liquid chromatography system, by Cytiva (2 mentions)

Close spelling variants of this product

HiTrap MabSelect PrismA™ HiTrap MabSelect column MabSelect PrismA Protein A columns HiTrap columns

2 protocols using hitrap mabselect prisma protein a column

Recombinant Human Antibody Production

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Heavy chain and light chain sequences of the complimentary determinant regions (CDRs) of Centi-C10 ScFv was cloned into each constant region of human IgG1 heavy chain, and human kappa constant IGKC, in the mammalian expression pTT5 vector (Licensed from National Research Council of Canada, Toronto Canada), respectively. Centi-C10 was generated in Transient Transfection using human embryonic kidney Expi293F™ ThermoFisher Scientific (San Diego, California), according to manufacture protocol at Centivax Inc. (South San Francisco). The antibody was then purified using HiTrap MabSelect PrismA protein A column (Cytiva, Marlborough, MA,), pre-equilibrated with at least 5 column volume (CV) of 1x PBS, pH 7.9 (ThermoFisher Scientific), in AKTA pure 25M fast protein liquid chromatography (FPLC) system (Cytiva). Flow rate was adjusted accordingly depending on the protein titer to achieve appropriate dynamic binding capacity.

Kasse C.M., Yu A.C., Powell A.E., Roth G.A., Liong C.S., Jons C.K., Buahin A., Maikawa C.L., Zhou X., Youssef S., Glanville J.E, & Appel E.A. (2023). Subcutaneous delivery of an antibody against SARS-CoV-2 from a supramolecular hydrogel depot. Biomaterials Science, 11(6), 2065-2079.

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Recombinant Human Antibody Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heavy chain and light chain sequences of the complimentary determinant regions (CDRs) of centi-C10 ScFv was cloned into each constant region of human IgG1 heavy chain, and human kappa constant IGKC, in the mammalian expression pTT5 vector (Licensed from National Research Council of Canada, Toronto Canada), respectively. Centi-C10 was generated in Transient Transfection using human embryonic kidney Expi293F^™ ThermoFisher Scientific (San Diego, California), according to manufacture protocol at Centivax Inc. (South San Francisco). The antibody was then purified using HiTrap MabSelect PrismA protein A column (Cytiva, Marlborough, MA,), pre-equilibrated with at least 5 column volume (CV) of 1x PBS, pH 7.9 (ThermoFisher Scientific), in AKTA pure 25M fast protein liquid chromatography (FPLC) system (Cytiva). Flow rate was adjusted accordingly depending on the protein titer to achieve appropriate dynamic binding capacity.

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